EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.19 | mutant Y167H is expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.19 | mutant Y167H, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.05 M Tris-HCl, 0.1 M sodium acetate buffer, 25 mM Na2HPO4, 150 mM NaCl, 10 mM imidazole pH 8.5 | Paenibacillus macerans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.19 | Y167H | the mutant shows enhanced alpha-cyclodextrin specificity | Paenibacillus macerans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.19 | 66000 | - |
x * 66000, SDS-PAGE | Paenibacillus macerans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.19 | starch + glycosyl acceptor | Paenibacillus macerans | - |
cyclodextrins | - |
? | |
2.4.1.19 | starch + glycosyl acceptor | Paenibacillus macerans 602-1 | - |
cyclodextrins | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.19 | Paenibacillus macerans | S5ZJ19 | - |
- |
2.4.1.19 | Paenibacillus macerans 602-1 | S5ZJ19 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.19 | mutant Y167H with ammonium sulfate precipitation and Ni-NTA agarose column chromatography | Paenibacillus macerans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.19 | starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
? | |
2.4.1.19 | starch + glycosyl acceptor | - |
Paenibacillus macerans 602-1 | cyclodextrins | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.19 | ? | x * 66000, SDS-PAGE | Paenibacillus macerans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.19 | alpha-cyclodextrin glucanotransferase | - |
Paenibacillus macerans |
2.4.1.19 | CGTase | - |
Paenibacillus macerans |