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Literature summary extracted from

  • Yue, Y.; Liu, S.; Li, H.; Song, B.; Xie, T.; Sun, Y.; Chao, Y.; Qian, S.
    Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans (2013), Acta Crystallogr. Sect. F, 69, 1186-1189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 mutant Y167H is expressed in Escherichia coli BL21(DE3) cells Paenibacillus macerans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.19 mutant Y167H, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.05 M Tris-HCl, 0.1 M sodium acetate buffer, 25 mM Na2HPO4, 150 mM NaCl, 10 mM imidazole pH 8.5 Paenibacillus macerans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 Y167H the mutant shows enhanced alpha-cyclodextrin specificity Paenibacillus macerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 66000
-
x * 66000, SDS-PAGE Paenibacillus macerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.19 starch + glycosyl acceptor Paenibacillus macerans
-
cyclodextrins
-
?
2.4.1.19 starch + glycosyl acceptor Paenibacillus macerans 602-1
-
cyclodextrins
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans S5ZJ19
-
-
2.4.1.19 Paenibacillus macerans 602-1 S5ZJ19
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 mutant Y167H with ammonium sulfate precipitation and Ni-NTA agarose column chromatography Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 starch + glycosyl acceptor
-
Paenibacillus macerans cyclodextrins
-
?
2.4.1.19 starch + glycosyl acceptor
-
Paenibacillus macerans 602-1 cyclodextrins
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 66000, SDS-PAGE Paenibacillus macerans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 alpha-cyclodextrin glucanotransferase
-
Paenibacillus macerans
2.4.1.19 CGTase
-
Paenibacillus macerans