Literature summary extracted from

Adachi, T.; Izumi, A.; Rea, D.; Park, S.Y.; Tame, J.R.; Roper, D.I.
Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C (2006), Acta Crystallogr. Sect. F, 62, 1010-1012.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.163	expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.2.1.163	sitting drop vapor diffusion method, using 6.4% (w/v) polyethylene glycol 20000, 5% (w/v) polyethylene glycol 550 monomethyl ether, 0.7 M potassium thiocyanate, in 50 mM MES pH 5.5	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.2.1.163	(4Z)-2-oxohept-4-enedioate + H2O	Escherichia coli	-	(4S)-4-hydroxy-2-oxoheptanedioate	-	r
4.2.1.163	(4Z)-2-oxohept-4-enedioate + H2O	Escherichia coli C	-	(4S)-4-hydroxy-2-oxoheptanedioate	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.163	Escherichia coli	P42270	-	-
4.2.1.163	Escherichia coli C	P42270	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.1.163	ammonium sulfate precipitation, HisTrap column chromatography, and Superdex 75 gel filtration	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.163	(4Z)-2-oxohept-4-enedioate + H2O	-	Escherichia coli	(4S)-4-hydroxy-2-oxoheptanedioate	-	r
4.2.1.163	(4Z)-2-oxohept-4-enedioate + H2O	-	Escherichia coli C	(4S)-4-hydroxy-2-oxoheptanedioate	-	r

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.163	HpcG	-	Escherichia coli
4.2.1.163	OHED hydratase	-	Escherichia coli

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Release 2023.1 (January 2023)