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Literature summary extracted from

  • Safonova, T.N.; Mikhailov, S.N.; Veiko, V.P.; Mordkovich, N.N.; Manuvera, V.A.; Alekseev, C.S.; Kovalchuk, M.V.; Popov, V.O.; Polyakov, K.M.
    High-syn conformation of uridine and asymmetry of the hexameric molecule revealed in the high-resolution structures of Shewanella oneidensis MR-1 uridine phosphorylase in the free form and in complex with uridine (2014), Acta Crystallogr. Sect. D, 70, 3310-3319.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 free enzyme and in complex with uridine, hanging drop vapor diffusion method, using 0.75 M ammonium sulfate, 0.075 M bis-Tris pH 5.5, 0.75% (w/v) PEG 3350, and 25% (v/v) glycerol Shewanella oneidensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 27000
-
6 * 27000, SDS-PAGE Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Shewanella oneidensis
-
uracil + alpha-D-ribose 1-phosphate
-
?
2.4.2.3 uridine + phosphate Shewanella oneidensis MR-1 / ATCC 700550
-
uracil + alpha-D-ribose 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Shewanella oneidensis C7EWM3
-
-
2.4.2.3 Shewanella oneidensis MR-1 / ATCC 700550 C7EWM3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 uridine + phosphate
-
Shewanella oneidensis uracil + alpha-D-ribose 1-phosphate
-
?
2.4.2.3 uridine + phosphate
-
Shewanella oneidensis MR-1 / ATCC 700550 uracil + alpha-D-ribose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.3 homohexamer 6 * 27000, SDS-PAGE Shewanella oneidensis