EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.176 | degradation | comparison of the activity w ith Humicola jecorina Cel7A reveals a much higher hydrolytic rate for Humicola grisea Cel7A at both 65°C (4.8fold higher initial rate) and 38°C (3.3fold higher) | [Humicola] grisea var. thermoidea |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.176 | protein lacking the C-terminal linker-CBM1 part, yielding crystals of space group P212121 with two protein molecules, chains A and B, in the asymmetric unit, to 1.8 A resolution. The catalytic triad consisting of Glu213 (nucleophile), Asp215 and Glu218 (acid/base), and the tryptophan platforms at subsites -7, -4, -2 and +1 are highly conserved | [Humicola] grisea var. thermoidea |
3.2.1.176 | structure of catalytic module, 1.8 A resolution. The crystal structure of the enzyme reveals considerable flexibility of the active-site-defining loop regions | [Humicola] grisea var. thermoidea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.176 | [Humicola] grisea var. thermoidea | P15828 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.176 | 4-methylumbelliferyl-beta-D-lactoside + H2O | - |
[Humicola] grisea var. thermoidea | 4-methylumbelliferol + D-lactose | - |
? | |
3.2.1.176 | cellulose + H2O | substrate acid-swollen cellulose | [Humicola] grisea var. thermoidea | cellobiose + ? | - |
? | |
3.2.1.176 | corn stover + H2O | - |
[Humicola] grisea var. thermoidea | cellobiose + ? | - |
? | |
3.2.1.176 | pretreated corn stover + H2O | - |
[Humicola] grisea var. thermoidea | ? | 14% conversion, 24 h, 65°C, best performance of the tested enzymes | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.176 | CBH-1 | - |
[Humicola] grisea var. thermoidea |
3.2.1.176 | Cel7A | - |
[Humicola] grisea var. thermoidea |
3.2.1.176 | exoglucanase-1 | - |
[Humicola] grisea var. thermoidea |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.176 | 72.5 | - |
melting temperature | [Humicola] grisea var. thermoidea |