Literature summary extracted from

Boeth, D.; Steiner, E.M.; Stadler, D.; Lindqvist, Y.; Schnell, R.; Schneider, G.
Structure of LdtMt2, an L,D-transpeptidase from Mycobacterium tuberculosis (2013), Acta Crystallogr. Sect. D, 69, 432-441.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.2.12	expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.3.2.12	sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M bis-Tris-HCl pH 6.5, 25% (w/v) PEG 3350	Mycobacterium tuberculosis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.2.12	peptidyl-tRNA1 + aminoacyl-tRNA2	Mycobacterium tuberculosis	-	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?
2.3.2.12	peptidyl-tRNA1 + aminoacyl-tRNA2	Mycobacterium tuberculosis CDC 1551	-	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.2.12	Mycobacterium tuberculosis	O53223	-	-
2.3.2.12	Mycobacterium tuberculosis CDC 1551	O53223	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.2.12	Ni-NTA resin column chromatography and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.2.12	peptidyl-tRNA1 + aminoacyl-tRNA2	-	Mycobacterium tuberculosis	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?
2.3.2.12	peptidyl-tRNA1 + aminoacyl-tRNA2	-	Mycobacterium tuberculosis CDC 1551	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.3.2.12	L,D-transpeptidase	-	Mycobacterium tuberculosis
2.3.2.12	LdtMt2	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)