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Literature summary extracted from

  • Zhang, W.; Shrestha, R.; Buckley, R.M.; Jewell, J.; Bossmann, S.H.; Stubbe, J.; Li, P.
    Mechanistic insight with HBCH2CoA as a probe to polyhydroxybutyrate (PHB) synthases (2014), ACS Chem. Biol., 9, 1773-1779.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.304 HBCH2CoA substrate analogue, in which the S-atom in hydroxybutanoyl-CoA is replaced with a CH2 group. HBCH2CoA is a competitive inhibitor of class I and class III PHB synthases. Upon incubation with a synthase acylated with a [3H]-saturated trimer-CoA, i.e. sTCoA, products are the methylene analogue of [3H]-sTCoA, [3H]-sT-CH2-CoA, saturated dimer-[3H]-sD-CO2H, and trimer-acid [3H]-sT-CO2H. HBCH2CoA may be reporting on the termination and repriming process of the synthases, rather than elongation Allochromatium vinosum
2.3.1.304 HBCH2CoA substrate analogue, in which the S-atom in hydroxybutanoyl-CoA is replaced with a CH2 group. HBCH2CoA is a competitive inhibitor of class I and class III PHB synthases. Upon incubation with a synthase acylated with a [3H]-saturated trimer-CoA, i.e. sTCoA, products are the methylene analogue of [3H]-sTCoA, [3H]-sT-CH2-CoA, saturated dimer-[3H]-sD-CO2H, and trimer-acid [3H]-sT-CO2H. HBCH2CoA may be reporting on the termination and repriming process of the synthases, rather than elongation Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.304 3.3
-
 (R)-3-hydroxybutanoyl-CoA pH 7.2, 25°C, presence of 0.05% Hecameg Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Allochromatium vinosum
-
-
-
2.3.1.304 Cupriavidus necator
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n
-
 Cupriavidus necator [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n
-
 Allochromatium vinosum [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.304 196
-
 (R)-3-hydroxybutanoyl-CoA pH 7.2, 25°C, presence of 0.05% Hecameg Cupriavidus necator

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.304 0.014
-
 HBCH2CoA pH 7.2, 37°C Allochromatium vinosum
2.3.1.304 0.04
-
 HBCH2CoA pH 7.2, 25°C Cupriavidus necator