BRENDA - Enzyme Database show

Nicotinamidase/pyrazinamidase of Mycobacterium tuberculosis forms homo-dimers stabilized by disulfide bonds

Rueda, D.; Sheen, P.; Gilman, R.H.; Bueno, C.; Santos, M.; Pando-Robles, V.; Batista, C.V.; Zimic, M.; Tuberculosis 94, 644-648 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.B15
gene pncA, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain BL21(DE3)pLysS
Mycobacterium tuberculosis
3.5.1.19
gene pncA, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain BL21(DE3)pLysS
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.B15
39700
-
dimeric enzyme, gel filtration
Mycobacterium tuberculosis
3.5.1.19
39700
-
dimeric enzyme, gel filtration
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.B15
nicotinamide + H2O
Mycobacterium tuberculosis
-
nicotinate + NH3
-
-
?
3.5.1.B15
nicotinamide + H2O
Mycobacterium tuberculosis H37Rv
-
nicotinate + NH3
-
-
?
3.5.1.B15
pyrazinamide + H2O
Mycobacterium tuberculosis
-
pyrazinoic acid + NH3
-
-
?
3.5.1.B15
pyrazinamide + H2O
Mycobacterium tuberculosis H37Rv
-
pyrazinoic acid + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Mycobacterium tuberculosis
-
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Mycobacterium tuberculosis H37Rv
-
nicotinate + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Mycobacterium tuberculosis
-
pyrazinoic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Mycobacterium tuberculosis H37Rv
-
pyrazinoic acid + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.B15
Mycobacterium tuberculosis
I6XD65
gene pncA
-
3.5.1.B15
Mycobacterium tuberculosis H37Rv
I6XD65
gene pncA
-
3.5.1.19
Mycobacterium tuberculosis
I6XD65
gene pncA
-
3.5.1.19
Mycobacterium tuberculosis H37Rv
I6XD65
gene pncA
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.B15
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration
Mycobacterium tuberculosis
3.5.1.19
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.B15
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis
nicotinate + NH3
-
-
-
?
3.5.1.B15
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
nicotinate + NH3
-
-
-
?
3.5.1.B15
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis
pyrazinoic acid + NH3
-
-
-
?
3.5.1.B15
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
pyrazinoic acid + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.B15
homodimer
2 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, structural model of enzyme homodimer, overview
Mycobacterium tuberculosis
3.5.1.B15
monomer
1 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, overview
Mycobacterium tuberculosis
3.5.1.19
homodimer
2 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, structural model of enzyme homodimer, overview
Mycobacterium tuberculosis
3.5.1.19
monomer
1 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, overview
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.B15
gene pncA, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain BL21(DE3)pLysS
Mycobacterium tuberculosis
3.5.1.19
gene pncA, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain BL21(DE3)pLysS
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.B15
39700
-
dimeric enzyme, gel filtration
Mycobacterium tuberculosis
3.5.1.19
39700
-
dimeric enzyme, gel filtration
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.B15
nicotinamide + H2O
Mycobacterium tuberculosis
-
nicotinate + NH3
-
-
?
3.5.1.B15
nicotinamide + H2O
Mycobacterium tuberculosis H37Rv
-
nicotinate + NH3
-
-
?
3.5.1.B15
pyrazinamide + H2O
Mycobacterium tuberculosis
-
pyrazinoic acid + NH3
-
-
?
3.5.1.B15
pyrazinamide + H2O
Mycobacterium tuberculosis H37Rv
-
pyrazinoic acid + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Mycobacterium tuberculosis
-
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Mycobacterium tuberculosis H37Rv
-
nicotinate + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Mycobacterium tuberculosis
-
pyrazinoic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Mycobacterium tuberculosis H37Rv
-
pyrazinoic acid + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.B15
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration
Mycobacterium tuberculosis
3.5.1.19
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and gel filtration
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.B15
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis
nicotinate + NH3
-
-
-
?
3.5.1.B15
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
nicotinate + NH3
-
-
-
?
3.5.1.B15
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis
pyrazinoic acid + NH3
-
-
-
?
3.5.1.B15
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
735324
Mycobacterium tuberculosis H37Rv
pyrazinoic acid + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.B15
homodimer
2 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, structural model of enzyme homodimer, overview
Mycobacterium tuberculosis
3.5.1.B15
monomer
1 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, overview
Mycobacterium tuberculosis
3.5.1.19
homodimer
2 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, structural model of enzyme homodimer, overview
Mycobacterium tuberculosis
3.5.1.19
monomer
1 * 20500-20700, about, recombinant enzyme, SDS-PAGE and mass spectrometry. Homodimers show a slightly lower specific enzyme activity compared to monomers. Enzyme dimers are dissociated into monomers in response to reducing conditions, disulfide bonds C72-C138 and C138-C138 stabilize the quaternary structure of the enzyme homodimer, quarternary structure analysis, overview
Mycobacterium tuberculosis
General Information
EC Number
General Information
Commentary
Organism
3.5.1.B15
additional information
structural modeling of the enzyme homodimer, docking study and molecular dynamics simulations, overview
Mycobacterium tuberculosis
3.5.1.19
additional information
structural modeling of the enzyme homodimer, docking study and molecular dynamics simulations, overview
Mycobacterium tuberculosis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.B15
additional information
structural modeling of the enzyme homodimer, docking study and molecular dynamics simulations, overview
Mycobacterium tuberculosis
3.5.1.19
additional information
structural modeling of the enzyme homodimer, docking study and molecular dynamics simulations, overview
Mycobacterium tuberculosis