BRENDA - Enzyme Database

Expression and functional characterization of a C-7 cholesterol desaturase from Tetrahymena thermophila in an insect cell line

Poklepovich, T.; Urtasun, N.; Miranda, M.; Nusblat, A.; Nudel, C.; Steroids 96, 132-139 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.21
expressed in Sf9 insect cells
Tetrahymena thermophila
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.21
membrane
transmembrane protein
Tetrahymena thermophila
16020
-
1.14.19.21
microsome
-
Tetrahymena thermophila
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.21
63000
-
x * 63000, SDS-PAGE
Tetrahymena thermophila
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.21
22-cholesten-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
22-cholesten-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholestan-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholestan-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.21
Tetrahymena thermophila
I7ML19
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.21
22-cholesten-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
22-cholesten-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholestan-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholestan-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.21
?
x * 63000, SDS-PAGE
Tetrahymena thermophila
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
NADH
-
Tetrahymena thermophila
1.14.19.21
NADPH
-
Tetrahymena thermophila
1.14.19.21
[2Fe-2S]-center
-
Tetrahymena thermophila
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.21
expressed in Sf9 insect cells
Tetrahymena thermophila
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
NADH
-
Tetrahymena thermophila
1.14.19.21
NADPH
-
Tetrahymena thermophila
1.14.19.21
[2Fe-2S]-center
-
Tetrahymena thermophila
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.21
membrane
transmembrane protein
Tetrahymena thermophila
16020
-
1.14.19.21
microsome
-
Tetrahymena thermophila
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.21
63000
-
x * 63000, SDS-PAGE
Tetrahymena thermophila
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.21
22-cholesten-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
22-cholesten-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholestan-3beta-ol + NADH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholestan-3beta-ol + NADPH + H+
Tetrahymena thermophila
-
7-cholesten-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Tetrahymena thermophila
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.21
22-cholesten-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
22-cholesten-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholestan-3beta-ol + NADH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholestan-3beta-ol + NADPH + H+
-
735284
Tetrahymena thermophila
7-cholesten-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
735284
Tetrahymena thermophila
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.21
?
x * 63000, SDS-PAGE
Tetrahymena thermophila