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Literature summary extracted from

  • Poklepovich, T.; Urtasun, N.; Miranda, M.; Nusblat, A.; Nudel, C.
    Expression and functional characterization of a C-7 cholesterol desaturase from Tetrahymena thermophila in an insect cell line (2015), Steroids, 96, 132-139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.21 expressed in Sf9 insect cells Tetrahymena thermophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.21 membrane transmembrane protein Tetrahymena thermophila 16020
-
1.14.19.21 microsome
-
 Tetrahymena thermophila
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.21 63000
-
 x * 63000, SDS-PAGE Tetrahymena thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.21 22-cholesten-3beta-ol + NADH + H+ Tetrahymena thermophila
-
 7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
 ?
1.14.19.21 22-cholesten-3beta-ol + NADPH + H+ Tetrahymena thermophila
-
 7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
 ?
1.14.19.21 cholestan-3beta-ol + NADH + H+ Tetrahymena thermophila
-
 7-cholesten-3beta-ol + NAD+ + H2O
-
 ?
1.14.19.21 cholestan-3beta-ol + NADPH + H+ Tetrahymena thermophila
-
 7-cholesten-3beta-ol + NADP+ + H2O
-
 ?
1.14.19.21 cholesterol + O2 + NADH + H+ Tetrahymena thermophila
-
 cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
 ?
1.14.19.21 cholesterol + O2 + NADPH + H+ Tetrahymena thermophila
-
 cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.21 Tetrahymena thermophila I7ML19
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.21 22-cholesten-3beta-ol + NADH + H+
-
 Tetrahymena thermophila 7,22-cholestadiene-3beta-ol + NAD+ + H2O
-
 ?
1.14.19.21 22-cholesten-3beta-ol + NADPH + H+
-
 Tetrahymena thermophila 7,22-cholestadiene-3beta-ol + NADP+ + H2O
-
 ?
1.14.19.21 cholestan-3beta-ol + NADH + H+
-
 Tetrahymena thermophila 7-cholesten-3beta-ol + NAD+ + H2O
-
 ?
1.14.19.21 cholestan-3beta-ol + NADPH + H+
-
 Tetrahymena thermophila 7-cholesten-3beta-ol + NADP+ + H2O
-
 ?
1.14.19.21 cholesterol + O2 + NADH + H+
-
 Tetrahymena thermophila cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
 ?
1.14.19.21 cholesterol + O2 + NADPH + H+
-
 Tetrahymena thermophila cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.21 ? x * 63000, SDS-PAGE Tetrahymena thermophila

Synonyms

EC Number Synonyms Comment Organism
1.14.19.21 C-7 cholesterol desaturase
-
 Tetrahymena thermophila
1.14.19.21 Des7
-
 Tetrahymena thermophila
1.14.19.21 Des7p
-
 Tetrahymena thermophila

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.21 NADH
-
 Tetrahymena thermophila
1.14.19.21 NADPH
-
 Tetrahymena thermophila
1.14.19.21 [2Fe-2S]-center
-
 Tetrahymena thermophila