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Literature summary extracted from

  • Hemsworth, G.R.; Price, H.P.; Smith, D.F.; Wilson, K.S.
    Crystal structure of the small GTPase Arl6/BBS3 from Trypanosoma brucei (2013), Protein Sci., 22, 196-203.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.2 recombinant expression of N-terminally His6-tagged TbArl6 Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.2 purified enzyme complexed with non-hydrolysable GTP analog GppNp, mixing of 8 mM guanosine 5'-[beta,gamma-imido]triphosphate (GppNp) and 92 mg/ml TbArl6 protein are mixed with 0.2 M CsCl and 2.2 M (NH4)2SO4, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement using the human Arl6 structure, PDB ID 2h57, as the search model Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.5.2 Mg2+ required Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.5.2 21400
-
 enzyme with bound GppNp, gel filtration Trypanosoma brucei
3.6.5.2 21700
-
 enzyme with bound GDPs, gel filtration Trypanosoma brucei
3.6.5.2 21800
-
 multi-angle laser light scattering and sequence calculation Trypanosoma brucei
3.6.5.2 21803
-
 1 * 21803, multi-angle laser light scattering and sequence calculation Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.2 GTP + H2O Trypanosoma brucei
-
 GDP + phosphate
-
 ?
3.6.5.2 additional information Trypanosoma brucei the enzyme interacts with tubulin and co-localises to the flagellar pocket with BBS1 when this protein is overexpressed ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.2 Trypanosoma brucei C9ZVV9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.5.2 lipoprotein the enzyme is N-myristoylated Trypanosoma brucei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.2 recombinant N-terminally His6-tagged TbArl6 by affinity chromatgraphy and gelfiltration Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.2 GTP + H2O
-
 Trypanosoma brucei GDP + phosphate
-
 ?
3.6.5.2 GTP + H2O nucleotide binding pocket and binding structures, overview Trypanosoma brucei GDP + phosphate
-
 ?
3.6.5.2 additional information the enzyme interacts with tubulin and co-localises to the flagellar pocket with BBS1 when this protein is overexpressed Trypanosoma brucei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.5.2 monomer 1 * 21803, multi-angle laser light scattering and sequence calculation Trypanosoma brucei
3.6.5.2 More structure of TbArl6, modeling, overview Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
3.6.5.2 Arl6/BBS3
-
 Trypanosoma brucei
3.6.5.2 small GTPase
-
 Trypanosoma brucei
3.6.5.2 TbArl6
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
3.6.5.2 malfunction RNAi knockdown of Trypanosom brucei Arl6 results in shortening of the trypanosome flagellum without loss of motility Trypanosoma brucei
3.6.5.2 additional information structure analysis and comparison of trypanosomal and human Arl6/BBS3 proteins, overview. Analysis of the TbArl6 active site confirms that it lacks the key glutamine that activates the nucleophile during GTP hydrolysis in other small GTPases. The trypanosomal proteins are significantly shorter at their N-termini suggesting a different method of membrane insertion compared to humans. Two surface patches may be important for protein-protein interactions Trypanosoma brucei