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Literature summary extracted from

  • Kumar, A.; Pillay, B.; Olaniran, A.O.
    Cloning, expression, purification and three-dimensional structure prediction of haloalkane dehalogenase from a recently isolated Ancylobacter aquaticus strain UV5 (2014), Protein Expr. Purif., 99, 10-17.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 gene dhlA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Escherichia coli strain BL21(DE3) Ancylobacter aquaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.5 SDS 76% inhibition at 1%, 94% at 5% Ancylobacter aquaticus
3.8.1.5 Triton X100 35% inhibition at 1%, 83% at 5% Ancylobacter aquaticus
3.8.1.5 Tween 80 26% inhibition at 1%, 75% at 5% Ancylobacter aquaticus
3.8.1.5 Urea 79% inhibitin at 4 M, 93% at 8 M Ancylobacter aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 additional information
-
 additional information Michaelis-Menten kinetics Ancylobacter aquaticus
3.8.1.5 0.842
-
 1,2-Dichloroethane pH 9.0, 37°C, recombinant enzyme Ancylobacter aquaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.8.1.5 additional information no effect by EDTA, Ca2+, Cu2+, Fe2+, Mn2+, Mg2+, and Zn2+ at 1-5 mM Ancylobacter aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.5 35000
-
 x * 35000, recombinant enzyme, SDS-PAGE, x * 35144, sequence calculation Ancylobacter aquaticus
3.8.1.5 35144
-
 x * 35000, recombinant enzyme, SDS-PAGE, x * 35144, sequence calculation Ancylobacter aquaticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 1,2-dichloroethane + H2O Ancylobacter aquaticus
-
 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O Ancylobacter aquaticus UV5
-
 2-chloroethanol + chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Ancylobacter aquaticus B8YPW8 gene dhlA
-
3.8.1.5 Ancylobacter aquaticus UV5 B8YPW8 gene dhlA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 recombinant enzyme 28.1fold from Escherichia coli strain BL21(DE3) by ultrafiltration, anion exchange chromatography, and gel filtration Ancylobacter aquaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.5 92.3
-
 purified recombinant enzyme, substrate 1,2-dichloroethane, pH 9.0, 37°C Ancylobacter aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,2-dichloroethane + H2O
-
 Ancylobacter aquaticus 2-chloroethanol + chloride
-
 ?
3.8.1.5 1,2-dichloroethane + H2O
-
 Ancylobacter aquaticus UV5 2-chloroethanol + chloride
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 ? x * 35000, recombinant enzyme, SDS-PAGE, x * 35144, sequence calculation Ancylobacter aquaticus
3.8.1.5 More three-dimensional enzyme structure analysis modeling Ancylobacter aquaticus

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DhlA
-
 Ancylobacter aquaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.5 37
-
-
 Ancylobacter aquaticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.5 25 65 activity range, profile overview Ancylobacter aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.5 37
-
 purified recombinant enzyme, loss of 20% activity within 24 h Ancylobacter aquaticus
3.8.1.5 50
-
 purified recombinant enzyme retains approximately 50% of its activity after 1 h of incubation at 50°C, inactivation after 4 h Ancylobacter aquaticus
3.8.1.5 70
-
 purified recombinant enzyme inactivation within 1 h Ancylobacter aquaticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.5 2.37
-
 1,2-Dichloroethane pH 9.0, 37°C, recombinant enzyme Ancylobacter aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 9
-
-
 Ancylobacter aquaticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.5 3 11 activity range, profile overview Ancylobacter aquaticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.8.1.5 Ancylobacter aquaticus sequence calculation
-
 4.65

General Information

EC Number General Information Comment Organism
3.8.1.5 evolution the enzyme belongs to the alpha/beta hydrolase family with a catalytic triad composed of Asp-His-Asp in its active site Ancylobacter aquaticus
3.8.1.5 additional information three-dimensional enzyme structure analysis modeling Ancylobacter aquaticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.8.1.5 2.8
-
 1,2-Dichloroethane pH 9.0, 37°C, recombinant enzyme Ancylobacter aquaticus