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Literature summary extracted from

  • Liao, L.; Sun, X.; Zeng, Y.; Luo, W.; Yu, Y.; Chen, B.
    A new L-haloacid dehalogenase from the Arctic psychrotrophic Pseudoalteromonas sp. BSW20308 (2015), Polar Biol., 38, 1161-1169.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.8.1.2 additional information the L-haloacid dehalogenase from a psychrotrophic Pseudoalteromonas from the Arctic revealed its potential in industrial applications such as in synthetic chemistry and environmental protection Pseudoalteromonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 phylogenetic analysis, recombinant expression of N-terminally His- and S-tagged enzyme in Escherichia coli strain BMRosetta Pseudoalteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 0.15
-
monobromoacetic acid pH 8.2, 25°C Pseudoalteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 27800
-
x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation Pseudoalteromonas sp.
3.8.1.2 32500
-
x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation Pseudoalteromonas sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.8.1.2 acetonitrile 20-40% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.
3.8.1.2 DMSO about 92% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.
3.8.1.2 Ethanol 20-40% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.
3.8.1.2 Methanol 20-40% of activity remains after incubation in 40% of DMSO Pseudoalteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudoalteromonas sp.
-
isolated from the Arctic marine environment
-
3.8.1.2 Pseudoalteromonas sp. BSW20308
-
isolated from the Arctic marine environment
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2 recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity Pseudoalteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-bromopropionic acid + H2O 41% activity compared to monobromoacetic acid Pseudoalteromonas sp. (R)-2-hydroxypropionic acid + bromide
-
?
3.8.1.2 (S)-2-bromopropionic acid + H2O 41% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 (R)-2-hydroxypropionic acid + bromide
-
?
3.8.1.2 (S)-2-chlorobutyric acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. (R)-2-hydroxybutyric acid + chloride
-
?
3.8.1.2 (S)-2-chlorobutyric acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 (R)-2-hydroxybutyric acid + chloride
-
?
3.8.1.2 (S)-2-chloropropionic acid + H2O 14% activity compared to monobromoacetic acid Pseudoalteromonas sp. (R)-2-hydroxypropionic acid + chloride
-
?
3.8.1.2 (S)-2-chloropropionic acid + H2O 14% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 (R)-2-hydroxypropionic acid + chloride
-
?
3.8.1.2 dichloroacetic acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. ?
-
?
3.8.1.2 dichloroacetic acid + H2O below 10% activity compared to monobromoacetic acid Pseudoalteromonas sp. BSW20308 ?
-
?
3.8.1.2 monobromoacetic acid + H2O best substrate Pseudoalteromonas sp. acetic acid + bromide
-
?
3.8.1.2 monobromoacetic acid + H2O best substrate Pseudoalteromonas sp. BSW20308 acetic acid + bromide
-
?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 ? x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation Pseudoalteromonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.8.1.2 2-HAD
-
Pseudoalteromonas sp.
3.8.1.2 2-haloacid dehalogenase
-
Pseudoalteromonas sp.
3.8.1.2 2-haloalkanoate dehalogenase
-
Pseudoalteromonas sp.
3.8.1.2 HADIIBSW
-
Pseudoalteromonas sp.
3.8.1.2 L-HAD
-
Pseudoalteromonas sp.
3.8.1.2 L-haloacid dehalogenase
-
Pseudoalteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 40
-
-
Pseudoalteromonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.2 10 65 activity range, 20% of maximal activity at 10°C, about 80% at 60°C, inactivation above 65°C Pseudoalteromonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.2 40
-
purified recombinant enzyme, 2 h, completely stable up to Pseudoalteromonas sp.
3.8.1.2 60
-
purified recombinant enzyme, 2 h, 40% activity remaining Pseudoalteromonas sp.
3.8.1.2 65
-
purified recombinant enzyme, 2 h, inactivation Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 8.2
-
assay at Pseudoalteromonas sp.

General Information

EC Number General Information Comment Organism
3.8.1.2 evolution 2-HADs can be classified into two phylogenetically distinct groups according to their substrate specificity. Group II consists of only L-HADs, which selectively act on L-2-haloalkanoic acids to produce D-2-hydroxyacids. HADIIBSW is evolutionarily distinct from previously characterized L-HADs, sharing only 34% identity with L-HAD (DehRhb) from a Rhodobacteraceae family bacterium UDC319 as the closest relative Pseudoalteromonas sp.