EC Number | Application | Comment | Organism |
---|---|---|---|
3.8.1.2 | additional information | the L-haloacid dehalogenase from a psychrotrophic Pseudoalteromonas from the Arctic revealed its potential in industrial applications such as in synthetic chemistry and environmental protection | Pseudoalteromonas sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.8.1.2 | phylogenetic analysis, recombinant expression of N-terminally His- and S-tagged enzyme in Escherichia coli strain BMRosetta | Pseudoalteromonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.2 | 0.15 | - |
monobromoacetic acid | pH 8.2, 25°C | Pseudoalteromonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.8.1.2 | 27800 | - |
x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation | Pseudoalteromonas sp. |
3.8.1.2 | 32500 | - |
x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation | Pseudoalteromonas sp. |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.8.1.2 | acetonitrile | 20-40% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
3.8.1.2 | DMSO | about 92% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
3.8.1.2 | Ethanol | 20-40% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
3.8.1.2 | Methanol | 20-40% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.2 | Pseudoalteromonas sp. | - |
isolated from the Arctic marine environment | - |
3.8.1.2 | Pseudoalteromonas sp. BSW20308 | - |
isolated from the Arctic marine environment | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.8.1.2 | recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity | Pseudoalteromonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.2 | (S)-2-bromopropionic acid + H2O | 41% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | (R)-2-hydroxypropionic acid + bromide | - |
? | |
3.8.1.2 | (S)-2-bromopropionic acid + H2O | 41% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | (R)-2-hydroxypropionic acid + bromide | - |
? | |
3.8.1.2 | (S)-2-chlorobutyric acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | (R)-2-hydroxybutyric acid + chloride | - |
? | |
3.8.1.2 | (S)-2-chlorobutyric acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | (R)-2-hydroxybutyric acid + chloride | - |
? | |
3.8.1.2 | (S)-2-chloropropionic acid + H2O | 14% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | (R)-2-hydroxypropionic acid + chloride | - |
? | |
3.8.1.2 | (S)-2-chloropropionic acid + H2O | 14% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | (R)-2-hydroxypropionic acid + chloride | - |
? | |
3.8.1.2 | dichloroacetic acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | ? | - |
? | |
3.8.1.2 | dichloroacetic acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | ? | - |
? | |
3.8.1.2 | monobromoacetic acid + H2O | best substrate | Pseudoalteromonas sp. | acetic acid + bromide | - |
? | |
3.8.1.2 | monobromoacetic acid + H2O | best substrate | Pseudoalteromonas sp. BSW20308 | acetic acid + bromide | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.8.1.2 | ? | x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation | Pseudoalteromonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.8.1.2 | 2-HAD | - |
Pseudoalteromonas sp. |
3.8.1.2 | 2-haloacid dehalogenase | - |
Pseudoalteromonas sp. |
3.8.1.2 | 2-haloalkanoate dehalogenase | - |
Pseudoalteromonas sp. |
3.8.1.2 | HADIIBSW | - |
Pseudoalteromonas sp. |
3.8.1.2 | L-HAD | - |
Pseudoalteromonas sp. |
3.8.1.2 | L-haloacid dehalogenase | - |
Pseudoalteromonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.8.1.2 | 40 | - |
- |
Pseudoalteromonas sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.8.1.2 | 10 | 65 | activity range, 20% of maximal activity at 10°C, about 80% at 60°C, inactivation above 65°C | Pseudoalteromonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.8.1.2 | 40 | - |
purified recombinant enzyme, 2 h, completely stable up to | Pseudoalteromonas sp. |
3.8.1.2 | 60 | - |
purified recombinant enzyme, 2 h, 40% activity remaining | Pseudoalteromonas sp. |
3.8.1.2 | 65 | - |
purified recombinant enzyme, 2 h, inactivation | Pseudoalteromonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.8.1.2 | 8.2 | - |
assay at | Pseudoalteromonas sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.8.1.2 | evolution | 2-HADs can be classified into two phylogenetically distinct groups according to their substrate specificity. Group II consists of only L-HADs, which selectively act on L-2-haloalkanoic acids to produce D-2-hydroxyacids. HADIIBSW is evolutionarily distinct from previously characterized L-HADs, sharing only 34% identity with L-HAD (DehRhb) from a Rhodobacteraceae family bacterium UDC319 as the closest relative | Pseudoalteromonas sp. |