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Literature summary extracted from

  • Selao, T.; Zhang, L.; Ariöz, C.; Wieslander, A.; Norling, B.
    Subcellular localization of monoglucosyldiacylglycerol synthase in Synechocystis sp. PCC6803 and its unique regulation by lipid environment (2014), PLoS ONE, 9, e88153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.46 phosphatidylethanolamine mild activity-enhancing effect (60%, at 40 mol percent concentration) Synechocystis sp.
2.4.1.46 sulfoquinovosyldiacylglycerol most potent activator (100% increase of activity at 10 mol percent concentration) Synechocystis sp.
2.4.1.336 sulfoquinovosyldiacylglycerol 7fold stimulation by 40 mol% in mixed micelles Synechocystis sp.
2.4.1.336 sulfoquinovosyldiacylglycerol potently stimulates the enzyme activity Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.46 expressed in Escherichia coli BL21-DE3 Rosetta cells Synechocystis sp.
2.4.1.336 expression in Escherichia coli Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.46 digalactosyldiacylglycerol
-
 Synechocystis sp.
2.4.1.46 monogalactosyldiacylglycerol
-
 Synechocystis sp.
2.4.1.336 digalactosyldiacylglycerol inhibition by the final products of galactolipid synthesis Synechocystis sp.
2.4.1.336 monogalactosyldiacylglycerol inhibition by the final products of galactolipid synthesis Synechocystis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.46 plasma membrane
-
 Synechocystis sp. 5886
-
2.4.1.46 thylakoid membrane
-
 Synechocystis sp. 42651
-
2.4.1.336 membrane integral membrane protein Synechocystis sp. 16020
-
2.4.1.336 membrane plasma and thylakoid membranes Synechocystis sp. 16020
-
2.4.1.336 plasma membrane
-
 Synechocystis sp. 5886
-
2.4.1.336 thylakoid membrane Synechocystis sp. 9579
-
2.4.1.336 thylakoid membrane
-
 Synechocystis sp. 42651
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.46 UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol Synechocystis sp.
-
 UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
 ?
2.4.1.336 additional information Synechocystis sp. production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase ?
-
 ?
2.4.1.336 UDP-glucose + 1,2-diacyl-sn-glycerol Synechocystis sp.
-
 UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.46 Synechocystis sp.
-
-
-
2.4.1.336 Synechocystis sp. P74165
-
-
2.4.1.336 Synechocystis sp. P74165 gene sll1377
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.336 native enzyme from membranes fraction by ultracentrifugation Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.46 UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
 Synechocystis sp. UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
 ?
2.4.1.336 additional information production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase Synechocystis sp. ?
-
 ?
2.4.1.336 UDP-glucose + 1,2-diacyl-sn-glycerol
-
 Synechocystis sp. UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.46 MgdA
-
 Synechocystis sp.
2.4.1.46 MGS
-
 Synechocystis sp.
2.4.1.46 monoglucosyldiacylglycerol synthase
-
 Synechocystis sp.
2.4.1.46 Sll1377
-
 Synechocystis sp.
2.4.1.336 MgdA
-
 Synechocystis sp.
2.4.1.336 MGS
-
 Synechocystis sp.
2.4.1.336 monoglucosyldiacylglycerol synthase
-
 Synechocystis sp.
2.4.1.336 Sll1377
-
 Synechocystis sp.
2.4.1.336 UDP-glucose:1,2-diacylglycerol 3-beta-D-glucosyltransferase
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.336 30
-
 assay at Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.336 8
-
 assay at Synechocystis sp.

General Information

EC Number General Information Comment Organism
2.4.1.336 metabolism the enzyme is involved in the lipid synthesis in Synechocystis, pathway overview Synechocystis sp.
2.4.1.336 physiological function synthesis of monogalactosyldiacylglycerol and digalactosyldiacylglycerol, the major membrane lipids in cyanobacteria, begins with production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase Synechocystis sp.