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Literature summary extracted from

  • Sanchez-Carron, G.; Martinez-Monino, A.B.; Sola-Carvajal, A.; Takami, H.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    New insights into the phylogeny and molecular classification of nicotinamide mononucleotide deamidases (2013), PLoS ONE, 8, e82705.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.42 gene pncC, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) Oceanobacillus iheyensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.42 additional information
-
 additional information Michaelis-Menten kinetics Oceanobacillus iheyensis
3.5.1.42 0.18
-
 beta-nicotinamide D-ribonucleotide recombinant enzyme, pH 7.4, 37°C Oceanobacillus iheyensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.42 47320
-
 2 * 47320, recombinant His6-tagged enzyme, mass spectrometry Oceanobacillus iheyensis
3.5.1.42 96100
-
 recombinant His6-tagged enzyme, gel filtration Oceanobacillus iheyensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O Oceanobacillus iheyensis absolute specific for beta-nicotinate D-ribonucleotide + NH3
-
 ?
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O Oceanobacillus iheyensis HTE831 absolute specific for beta-nicotinate D-ribonucleotide + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.42 Oceanobacillus iheyensis Q8EQR8 gene pncC
-
3.5.1.42 Oceanobacillus iheyensis HTE831 Q8EQR8 gene pncC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.42 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration Oceanobacillus iheyensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O absolute specific for Oceanobacillus iheyensis beta-nicotinate D-ribonucleotide + NH3
-
 ?
3.5.1.42 beta-nicotinamide D-ribonucleotide + H2O absolute specific for Oceanobacillus iheyensis HTE831 beta-nicotinate D-ribonucleotide + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.42 homodimer 2 * 47320, recombinant His6-tagged enzyme, mass spectrometry Oceanobacillus iheyensis

Synonyms

EC Number Synonyms Comment Organism
3.5.1.42 nicotinamide mononucleotide deamidase
-
 Oceanobacillus iheyensis
3.5.1.42 NMN deamidase
-
 Oceanobacillus iheyensis
3.5.1.42 OiPncC
-
 Oceanobacillus iheyensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.42 65
-
-
 Oceanobacillus iheyensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.42 50
-
 purified recombinant enzyme, pH 7.3, stable up to Oceanobacillus iheyensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.42 0.38
-
 beta-nicotinamide D-ribonucleotide recombinant enzyme, pH 7.4, 37°C Oceanobacillus iheyensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.42 7.4
-
-
 Oceanobacillus iheyensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.42 5.5 10 the enzyme is active over a broad pH range, with an optimum at pH 7.4, whilst maintaining 90% activity at pH 10.0 Oceanobacillus iheyensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.42 10
-
 purified recombinant enzyme, 37°C, maintains 61% activity after 21 days Oceanobacillus iheyensis

General Information

EC Number General Information Comment Organism
3.5.1.42 metabolism nicotinamide mononucleotide (NMN) deamidase is one of the key enzymes of the bacterial pyridine nucleotide cycle, PNC Oceanobacillus iheyensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.42 2.1
-
 beta-nicotinamide D-ribonucleotide recombinant enzyme, pH 7.4, 37°C Oceanobacillus iheyensis