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Literature summary extracted from

  • Chen, Y.L.; Hu, N.T.
    Function-related positioning of the type II secretion ATPase of Xanthomonas campestris pv. campestris (2013), PLoS ONE, 8, e59123.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.4.2.8 additional information the cytoplasmic ATPase activity, when copurified with the cytoplasmic domain of an interactive ATPase partner, is stimulated by an acidic phospholipid Xanthomonas campestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.8 gene xpsE, recombinant expression of C-terminally ECFP-tagged enzyme in the xpsE-null strain XC1723 complementing the strain and restoring the secretion activity Xanthomonas campestris

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.8 additional information fluorescent-tagged ATPase expressed in secretion-proficient cells is mainly diffused in cytoplasm. Focal spots at the cell periphery are detectable only in a few cells. The discrete foci are augmented in abundance and intensity when the secretion channel is depleted and the exoprotein overproduced, overview. The foci abundance is inversely related to secretion efficiency of the secretion channel. Restored function of the secretion channel paralleles reduced ATPase foci abundance Xanthomonas campestris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.8 cytoplasm the T2S ATPase cycles between a cytoplasmic and a membrane-associated state in a secretion-related manner Xanthomonas campestris 5737
-
7.4.2.8 membrane the T2S ATPase cycles between a cytoplasmic and a membrane-associated state in a secretion-related manner, T2S ATPase may directly associate with the membrane Xanthomonas campestris 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.4.2.8 Mg2+ required Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.8 ATP + H2O Xanthomonas campestris
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.8 Xanthomonas campestris P31742 pv. campestris, gene xpsE
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.8 ATP + H2O
-
 Xanthomonas campestris ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.8 T2S ATPase
-
 Xanthomonas campestris
7.4.2.8 type II secretion ATPase
-
 Xanthomonas campestris
7.4.2.8 XpsE
-
 Xanthomonas campestris

General Information

EC Number General Information Comment Organism
7.4.2.8 metabolism Gram-negative bacteria use the type II secretion (T2S) system to secrete exoproteins for attacking animal or plant cells or to obtain nutrients from the environment. The system is unique in helping folded proteins traverse the outer membrane. The secretion machine comprises multiple proteins spanning the cell envelope and a cytoplasmic ATPase. Activity of the ATPase, when copurified with the cytoplasmic domain of an interactive ATPase partner, is stimulated by an acidic phospholipid, suggesting the membrane-associated ATPase is actively engaged in secretion Xanthomonas campestris
7.4.2.8 physiological function the enzyme ATPase foci colocalizes with the secretion channel. The ATPase may be transiently associated with the T2S machine by alternating between a cytoplasmic and a machine-associated state in a secretion-dependent manner, terminating the ATPase activity when secretion is completed. Function-related dynamic assembly may be the essence of the T2S machine, overview. The T2S ATPase acts as a transiently associated part of the secretion machine Xanthomonas campestris