Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eadsforth, T.C.; Gardiner, M.; Maluf, F.V.; McElroy, S.; James, D.; Frearson, J.; Gray, D.; Hunter, W.N.
    Assessment of Pseudomonas aeruginosa N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase as a potential antibacterial drug target (2012), PLoS ONE, 7, e35973.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.9 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.9 hanging drop vapor diffusion method, using 25% (w/v) PEG 3350 and 0.2 M magnesium formate Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.9 2-[(2-chloro-6-fluorobenzyl)sulfanyl]-6-(trifluoromethyl)pyrimidin-4-ol
-
Pseudomonas aeruginosa
3.5.4.9 5-[(4-chlorophenyl)sulfonyl]-6-ethoxypyrimidine-2,4-diamine
-
Pseudomonas aeruginosa
3.5.4.9 6-benzyl-5-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile
-
Pseudomonas aeruginosa
3.5.4.9 LY354899 i.e. 5,6,7,8-tetrahydro-N5,N10-carbonylfolic acid Pseudomonas aeruginosa
3.5.4.9 LY374571 i.e. (2R)-2-[(4-{[(2,5-diamino-6-hydroxypyrimidin-4-yl)carbamoyl]amino}phenyl)formamido]pentanedioic acid Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.9 0.026
-
5,10-methenyltetrahydrofolate at pH 7.9 and 22°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.9 30000
-
2 * 30000, SDS-PAGE Pseudomonas aeruginosa
3.5.4.9 60000
-
gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Pseudomonas aeruginosa
-
10-formyltetrahydrofolate
-
r

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.9 Pseudomonas aeruginosa Q9I2U6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.9 Ni2+-charged HisTrap column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
Pseudomonas aeruginosa 10-formyltetrahydrofolate
-
r

Subunits

EC Number Subunits Comment Organism
3.5.4.9 homodimer 2 * 30000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.4.9 FolD
-
Pseudomonas aeruginosa
3.5.4.9 N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase bifunctional enzyme Pseudomonas aeruginosa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.4.9 0.00003
-
at pH 7.9 and 22°C Pseudomonas aeruginosa LY374571
3.5.4.9 0.0009
-
at pH 7.9 and 22°C Pseudomonas aeruginosa 5-[(4-chlorophenyl)sulfonyl]-6-ethoxypyrimidine-2,4-diamine
3.5.4.9 0.0025
-
at pH 7.9 and 22°C Pseudomonas aeruginosa 2-[(2-chloro-6-fluorobenzyl)sulfanyl]-6-(trifluoromethyl)pyrimidin-4-ol
3.5.4.9 0.0038
-
at pH 7.9 and 22°C Pseudomonas aeruginosa 6-benzyl-5-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile