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Literature summary extracted from

  • Andreeva, N.; Trilisenko, L.; Eldarov, M.; Kulakovskaya, T.
    Polyphosphatase PPN1 of Saccharomyces cerevisiae: switching of exopolyphosphatase and endopolyphosphatase activities (2015), PLoS ONE, 10, e0119594.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.10 ADP ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+ Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.10 expression in Saccharomyces cerevisiae Saccharomyces cerevisiae
3.6.1.11 expression in Saccharomyces cerevisiae Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.10 ATP ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+ Saccharomyces cerevisiae
3.6.1.11 additional information in the presence of Mg2+, the enzyme shows a polyphosphate chain fragmentation activity. ATP inhibits while ADP activates endopolyphosphatase activity in the presence of Mg2+ Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.10 Mg2+ in the presence of Mg2+, the enzyme shows a polyphosphate chain fragmentation activity Saccharomyces cerevisiae
3.6.1.11 Co2+ Co2+ stimulates phosphate release from the polyphosphate chain end Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.10 Saccharomyces cerevisiae Q04119 bifunctional exo- and endopolyphosphatase, EC 3.6.1.11 and EC 3.6.1.10
-
3.6.1.11 Saccharomyces cerevisiae Q04119 bifunctional exo- and endopolyphosphatase, EC 3.6.1.11 and EC 3.6.1.10
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.10 proteolytic modification PPN1 gene encodes a polypeptide with the predicted molecular mass of 78 kDa, the monomer molecular mass of the mature PPN1 is about 33-35 kDa Saccharomyces cerevisiae
3.6.1.11 proteolytic modification PPN1 gene encodes a polypeptide with the predicted molecular mass of 78 kDa, the monomer molecular mass of the mature PPN1 is about 33-35 kDa Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.10
-
 Saccharomyces cerevisiae
3.6.1.11
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.11 3 8 substrate (phosphate)3, presence of 0.1 mM Co2+, pH 7.2, 30°C Saccharomyces cerevisiae
3.6.1.11 240
-
 substrate (phosphate)15, presence of 0.1 mM Co2+, pH 7.2, 30°C Saccharomyces cerevisiae
3.6.1.11 290
-
 substrate (phosphate)208, presence of 0.1 mM Co2+, pH 7.2, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.10 additional information enzyme is a bifunctional exo- and endopolyphosphatase, activities of ECs 3.6.1.11 and 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenyl phosphate Saccharomyces cerevisiae ?
-
 ?
3.6.1.11 (phosphate)15 + H2O
-
 Saccharomyces cerevisiae (phosphate)14 + phosphate
-
 ?
3.6.1.11 (phosphate)208 + H2O
-
 Saccharomyces cerevisiae (phosphate)207 + phosphate reaction continues to a chain length of about 15 residues ?
3.6.1.11 (phosphate)3 + H2O
-
 Saccharomyces cerevisiae (phosphate)2 + phosphate
-
 ?
3.6.1.11 additional information enzyme is a bifunctional exo- and endopolyphosphatase, activities of EC 3.6.1.11 and EC 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenylphosphate Saccharomyces cerevisiae ?
-
 ?