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Literature summary extracted from

  • Massalski, C.; Bloch, J.; Zebisch, M.; Steinebrunner, I.
    The biochemical properties of the Arabidopsis ecto-nucleoside triphosphate diphosphohydrolase AtAPY1 contradict a direct role in purinergic signaling (2015), PLoS ONE, 10, e0115832.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.5 expression of the full-length protein and a soluble form without the transmembrane domain near the N-terminus in HEK-293 cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 azide 10 mM, 42% inhibition Arabidopsis thaliana
3.6.1.5 Cu2+ 1 mM, about 3fold inhibition Arabidopsis thaliana
3.6.1.5 fluoride 10 mM, 27% inhibition Arabidopsis thaliana
3.6.1.5 vanadate 1 mM, 54% inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.0597
-
 GDP pH 6.5, 25°C Arabidopsis thaliana
3.6.1.5 0.0744
-
 UDP pH 6.5, 25°C Arabidopsis thaliana
3.6.1.5 0.166
-
 IDP pH 6.5, 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 membrane
-
 Arabidopsis thaliana 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ 1 mM, about 3fold stimulation. Ca2+ and Mn2+ are best activators Arabidopsis thaliana
3.6.1.5 Mg2+ 1 mM, 2.5fold stimulation Arabidopsis thaliana
3.6.1.5 Mn2+ 1 mM, about 3fold stimulation. Ca2+ and Mn2+ are best activators Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 52000
-
 x * 52000, recombinant protein with His-tag, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Arabidopsis thaliana Q9SQG2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 21.7
-
 recombinant protein lacking transmembrane domain, pH 6.5, 25°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 GDP + H2O
-
 Arabidopsis thaliana GMP + phosphate
-
 ?
3.6.1.5 IDP + H2O
-
 Arabidopsis thaliana IMP + phosphate
-
 ?
3.6.1.5 additional information no substrate: ADP Arabidopsis thaliana ?
-
 ?
3.6.1.5 UDP + H2O
-
 Arabidopsis thaliana UMP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 ? x * 52000, recombinant protein with His-tag, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.6.1.5 APY1
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.5 5.5 9.5 broad Arabidopsis thaliana