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Literature summary extracted from

  • Negi, V.S.; Bingham, J.P.; Li, Q.X.; Borthakur, D.
    A carbon-nitrogen lyase from Leucaena leucocephala catalyzes the first step of mimosine degradation (2014), Plant Physiol., 164, 922-934.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.61 gene seq3, DNA and amino acid sequence determination and analysis, codon optimization and recombinant expression in Escherichia coli Leucaena leucocephala

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.61 hydroxylamine complete inhibition at 0.05 mM, reversible by 100 nM pyridoxal 5'-phosphate Leucaena leucocephala
3.5.1.61 additional information no competitive inhibition by L-tyrosine, L-phenylalanine, and L-tryptophan Leucaena leucocephala

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.61 chloroplast the enzyme has a chloroplast transit peptide Leucaena leucocephala 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.61 45000
-
 x * 45000 recombinant enzyme, SDS-PAGE Leucaena leucocephala

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.61 (S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O Leucaena leucocephala
-
 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.61 Leucaena leucocephala A8CEI3 gene seq3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.61 recombinant enzyme from Escherichia coli Leucaena leucocephala

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.61 leaf
-
 Leucaena leucocephala
-
3.5.1.61 seedling
-
 Leucaena leucocephala
-
3.5.1.61 shoot
-
 Leucaena leucocephala
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.61 (S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
-
 Leucaena leucocephala 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3
-
 ?
3.5.1.61 (S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O i.e. mimosine, a toxic nonprotein aromatic amino acid. Dependence of the enzyme on pyridoxal 5'-phosphate and the production of 3-hydroxy-4H-pyrid-4-one with the release of ammonia indicate that the enzyme is a carbon-nitrogen lyase Leucaena leucocephala 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3 product identification by by electrospray ionization-tandem mass spectrometry ?

Subunits

EC Number Subunits Comment Organism
3.5.1.61 ? x * 45000 recombinant enzyme, SDS-PAGE Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.61 37
-
-
 Leucaena leucocephala

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.61 22 45 enzyme catalytic activity sharply decreases at 45°C or higher and below 22°C Leucaena leucocephala

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.61 40
-
 purified recombinant enzyme, pH 8.5, 37°C, around 80% activity remaining, fairly stable up to 40°C Leucaena leucocephala
3.5.1.61 50
-
 purified recombinant enzyme, pH 8.5, 37°C, the enzyme activity shows a sharp reduction Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.61 8.5
-
-
 Leucaena leucocephala

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.61 7 12 high catalytic activities at pH 7.5-10.0, inactive below pH 7.0 Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
3.5.1.61 additional information no requirement for an 2oxo acid Leucaena leucocephala
3.5.1.61 pyridoxal 5'-phosphate dependent on, enzyme-bound Leucaena leucocephala

General Information

EC Number General Information Comment Organism
3.5.1.61 metabolism the carbon-nitrogen lyase catalyzes the first step of mimosine degradation. Mimosine is a toxic nonprotein aromatic amino acid Leucaena leucocephala