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Literature summary extracted from

  • Mageroy, M.; Tieman, D.; Floystad, A.; Taylor, M.; Klee, H.
    A Solanum lycopersicum catechol-O-methyltransferase involved in synthesis of the flavor molecule guaiacol (2012), Plant J., 69, 1043-1051.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.6 expression in Escherichia coli Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.6 0.00836
-
catechol pH 7.5, 25°C Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.6 Solanum lycopersicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.6 catechol + S-adenosyl-L-methionine
-
Solanum lycopersicum guaiacol + S-adenosyl-L-homocysteine
-
?
2.1.1.6 additional information slight activity on orcinol, caffeic acid, and pyrogallol. No substrates: guaiacol, salicylic acid, benzoic acid, 2,5-dimethyl-4-methoxy-3(2H)-furanone Solanum lycopersicum ?
-
?
2.1.1.6 protocatechuic aldehyde + S-adenosyl-L-methionine 17% of the activity with catechol Solanum lycopersicum ? + S-adenosyl-L-homocysteine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.6 CTOMT1
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.6 9.67
-
catechol pH 7.5, 25°C Solanum lycopersicum

General Information

EC Number General Information Comment Organism
2.1.1.6 physiological function knockdown of isoform CTOMT1 results in significantly reduced fruit guaiacol emissions. CTOMT1 overexpression results in slightly increased fruit guaiacol emission. Upon supply with exogenously applied catechol, guaiacol production increases in both wild-type and transgenic fruit discs, although to a much greater extent in CTOMT1 overexpressing discs Solanum lycopersicum