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Literature summary extracted from

  • Fatihi, A.; Latimer, S.; Schmollinger, S.; Block, A.; Dussault, P.H.; Vermaas, W.F.; Merchant, S.S.; Basset, G.J.
    A dedicated type II NADPH dehydrogenase performs the penultimate step in the biosynthesis of vitamin K1 in Synechocystis and Arabidopsis (2015), Plant Cell, 27, 1730-1741.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.12 expressed in Escherichia coli BL21 Star (DE3) pLysS cells Synechocystis sp.
1.6.5.12 expressed in Escherichia coli BL21 Star (DE3) pLysS cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.12 dicumarol
-
 Arabidopsis thaliana
1.6.5.12 dicumarol
-
 Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.12 0.0024
-
 menadione pH 7.5, 30°C, recombinant enzyme Synechocystis sp.
1.6.5.12 0.02
-
 menadione pH 7.5, 30°C, recombinant enzyme Arabidopsis thaliana
2.1.1.329 0.02
-
 menadione at pH 7.5 and 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.12 chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.12 demethylphylloquinone + NADPH + H+ Synechocystis sp.
-
 demethylphylloquinol + NADP+
-
 ?
1.6.5.12 demethylphylloquinone + NADPH + H+ Arabidopsis thaliana
-
 demethylphylloquinol + NADP+
-
 ?
2.1.1.329 S-adenosyl-L-methionine + demethylphylloquinone Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + phylloquinone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.12 Arabidopsis thaliana Q8GXR9
-
-
1.6.5.12 Synechocystis sp.
-
-
-
2.1.1.329 Arabidopsis thaliana Q3ED65
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.12 Ni-NTA His-Bind resin column chromatography Synechocystis sp.
1.6.5.12 Ni-NTA His-Bind resin column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.12 demethylphylloquinone + NADPH + H+
-
 Synechocystis sp. demethylphylloquinol + NADP+
-
 ?
1.6.5.12 demethylphylloquinone + NADPH + H+
-
 Arabidopsis thaliana demethylphylloquinol + NADP+
-
 ?
1.6.5.12 menadione + NADPH + H+
-
 Synechocystis sp. menadiol + NADP+
-
 ?
1.6.5.12 menadione + NADPH + H+
-
 Arabidopsis thaliana menadiol + NADP+
-
 ?
2.1.1.329 S-adenosyl-L-methionine + demethylphylloquinone
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + phylloquinone
-
 ?
2.1.1.329 S-adenosyl-L-methionine + menadione
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.12 NAD(P)H dehydrogenase C1
-
 Arabidopsis thaliana
1.6.5.12 ndbB
-
 Synechocystis sp.
1.6.5.12 NDC1
-
 Arabidopsis thaliana
2.1.1.329 demethylnaphthoquinone methyltransferase
-
 Arabidopsis thaliana
2.1.1.329 menG
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.12 0.44
-
 menadione pH 7.5, 30°C, recombinant enzyme Arabidopsis thaliana
1.6.5.12 3.2
-
 menadione pH 7.5, 30°C, recombinant enzyme Synechocystis sp.
2.1.1.329 0.44
-
 menadione at pH 7.5 and 30°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.12 FAD
-
 Synechocystis sp.
1.6.5.12 FAD
-
 Arabidopsis thaliana
1.6.5.12 NADPH
-
 Synechocystis sp.
1.6.5.12 NADPH
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.5.12 0.0013
-
 dicumarol substrate menadione and NADPH Arabidopsis thaliana
1.6.5.12 0.0055
-
 dicumarol substrate menadione and NADPH Synechocystis sp.

General Information

EC Number General Information Comment Organism
1.6.5.12 malfunction enzyme deletion mutants display increased photosensitivity to high light Synechocystis sp.
1.6.5.12 malfunction enzyme deletion mutants display increased photosensitivity to high light Arabidopsis thaliana
1.6.5.12 metabolism the enzyme performs the penultimate step in the biosynthesis of vitamin K1 Synechocystis sp.
1.6.5.12 metabolism the enzyme performs the penultimate step in the biosynthesis of vitamin K1 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.329 22
-
 menadione at pH 7.5 and 30°C Arabidopsis thaliana