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Literature summary extracted from

  • Kajikawa, M.; Yamato, K.; Kohzu, Y.; Shoji, S.; Matsui, K.; Tanaka, Y.; Sakai, Y.; Fukuzawa, H.
    A front-end desaturase from Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by omega13 desaturation in methylotrophic yeast and tobacco (2006), Plant Cell Physiol., 47, 64-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.12 expressed in Pichia pastoris Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.12 alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii DELTA5-desaturase activity coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii 137C DELTA5-desaturase activity coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii low activity ?
-
 ?
1.14.19.12 dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii 137C low activity ?
-
 ?
1.14.19.12 eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii low activity ?
-
 ?
1.14.19.12 eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii 137C low activity ?
-
 ?
1.14.19.12 linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii preferred substrate, DELTA5-desaturase activity pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii 137C preferred substrate, DELTA5-desaturase activity pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 additional information Chlamydomonas reinhardtii no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively ?
-
 ?
1.14.19.12 additional information Chlamydomonas reinhardtii 137C no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively ?
-
 ?
1.14.19.12 oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ Chlamydomonas reinhardtii low activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.12 Chlamydomonas reinhardtii Q2HWK7
-
-
1.14.19.12 Chlamydomonas reinhardtii 137C Q2HWK7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.12 alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ DELTA5-desaturase activity Chlamydomonas reinhardtii coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ DELTA5-desaturase activity Chlamydomonas reinhardtii 137C coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ low activity Chlamydomonas reinhardtii ?
-
 ?
1.14.19.12 dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ low activity Chlamydomonas reinhardtii 137C ?
-
 ?
1.14.19.12 eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ low activity Chlamydomonas reinhardtii ?
-
 ?
1.14.19.12 eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ low activity Chlamydomonas reinhardtii 137C ?
-
 ?
1.14.19.12 linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ preferred substrate, DELTA5-desaturase activity Chlamydomonas reinhardtii pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ preferred substrate, DELTA5-desaturase activity Chlamydomonas reinhardtii 137C pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
-
 ?
1.14.19.12 additional information no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively Chlamydomonas reinhardtii ?
-
 ?
1.14.19.12 additional information no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively Chlamydomonas reinhardtii 137C ?
-
 ?
1.14.19.12 oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+ low activity Chlamydomonas reinhardtii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.12 DES
-
 Chlamydomonas reinhardtii
1.14.19.12 omega13 desaturase
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.12 cytochrome b5
-
 Chlamydomonas reinhardtii