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Literature summary extracted from

  • Zhao, N.; Yao, J.; Chaiprasongsuk, M.; Li, G.; Guan, J.; Tschaplinski, T.J.; Guo, H.; Chen, F.
    Molecular and biochemical characterization of the jasmonic acid methyltransferase gene from black cottonwood (Populus trichocarpa) (2013), Phytochemistry, 94, 74-81.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.141 N361S the mutation leads to higher specific activity with benzoate than with jasmonate Populus trichocarpa
2.1.1.141 S153Y the mutation leads to strongly reduced activity with benzoate or jasmonate Populus trichocarpa
2.1.1.141 S153Y/N361S the mutation leads to strongly reduced activity with benzoate or jasmonate Populus trichocarpa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.141 Ca2+ 5 mM Ca2+ shows a mild inhibition effect on enzyme activity (about 80% residual activity) Populus trichocarpa
2.1.1.141 Cu2+ complete inhibition at 5 mM Populus trichocarpa
2.1.1.141 Fe2+ complete inhibition at 5 mM Populus trichocarpa
2.1.1.141 Fe3+ complete inhibition at 5 mM Populus trichocarpa
2.1.1.141 Mg2+ shows a mild inhibition effect on enzyme activity (about 85% residual activity) Populus trichocarpa
2.1.1.141 Mn2+ 5 mM Mn2+ decreases the enzyme activity by 40% Populus trichocarpa
2.1.1.141 Zn2+ complete inhibition at 5 mM Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.141 0.0631
-
 benzoate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa
2.1.1.141 0.175
-
 jasmonate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa
2.1.1.141 0.31
-
 jasmonate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa
2.1.1.141 0.341
-
 benzoate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.141 K+ significantly stimulates enzyme activity (to about 250%) Populus trichocarpa
2.1.1.141 Na+ has a mild stimulation on enzyme activity (to about 160%) Populus trichocarpa
2.1.1.141 NH4+ has a mild stimulation on enzyme activity (to about 165%) Populus trichocarpa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.141 S-adenosyl-L-methionine + jasmonate Populus trichocarpa
-
 S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.141 Populus trichocarpa B9H4E1 black cottonwood
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.141 leaf
-
 Populus trichocarpa
-
2.1.1.141 seedling
-
 Populus trichocarpa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.141 additional information no activity with salicylic acid, indole-3-acetic acid, and gibberellic acid Populus trichocarpa ?
-
 ?
2.1.1.141 S-adenosyl-L-methionine + benzoate 22% activity compared to jasmonate Populus trichocarpa S-adenosyl-L-homocysteine + methyl benzoate
-
 ?
2.1.1.141 S-adenosyl-L-methionine + jasmonate
-
 Populus trichocarpa S-adenosyl-L-homocysteine + methyl jasmonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.141 jasmonic acid methyltransferase
-
 Populus trichocarpa
2.1.1.141 JMT1
-
 Populus trichocarpa
2.1.1.141 S-adenosyl-L-methionine:jasmonic acid carboxyl methyltransferase
-
 Populus trichocarpa
2.1.1.141 SABATH3 formerly Populus trichocarpa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.141 4 60 the enzyme is 100% stable for 30 min at 4°C. It loses about 10%, 20% and 40% of its maximal activity when incubated at 37°C, 42°C and 50°C, respectively for 30 min. When incubated at 60°C for 30 min, the enzyme loses all of its activity Populus trichocarpa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.141 0.003
-
 benzoate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa
2.1.1.141 0.013
-
 jasmonate wild type enzyme, at pH 6.5 and 30°C Populus trichocarpa
2.1.1.141 0.015
-
 jasmonate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa
2.1.1.141 0.028
-
 benzoate mutant enzyme N361S, at pH 6.5 and 30°C Populus trichocarpa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.141 6.5
-
-
 Populus trichocarpa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.141 6 8.5 at pH 6.0, pH 7.0, pH 8.0 and pH 8.5, the enzyme displays about 80%, 50%, 20% and 5% of its maximal activity, respectively Populus trichocarpa

Expression

EC Number Organism Comment Expression
2.1.1.141 Populus trichocarpa the enzyme expression is induced by methyl jasmonate and salicylic acid as well as alamethicin up

General Information

EC Number General Information Comment Organism
2.1.1.141 physiological function the enzyme plays a role in plant defense against biotic stresses Populus trichocarpa