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Literature summary extracted from

  • Wujak, M.; Banach, M.; Porowinska, D.; Piskulak, K.; Komoszynski, M.
    Isolation and bioinformatic analysis of seven genes encoding potato apyrase. Bacterial overexpresssion, refolding and initial kinetic studies on some recombinant potato apyrases (2013), Phytochemistry, 93, 8-17.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.5 isoforms APY4, APY5 and APY6 are expressed in Escherichia coli BL21(DE3) cells with thioredoxin-tag, His6-tag and S-tag Solanum tuberosum

General Stability

EC Number General Stability Organism
3.6.1.5 the removal of thioredoxin-tag from purified apyrase causes 10times reduction of specific activity Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 Cu2+
-
 Solanum tuberosum
3.6.1.5 EDTA complete inhibition at 10 mM Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+ best activator of isoform APY6 Solanum tuberosum
3.6.1.5 Co2+ activator of isoform APY6 Solanum tuberosum
3.6.1.5 Mg2+ an increase in ADPase activity in presence of Mg2+ ions occurs in case of isoform APY5 Solanum tuberosum
3.6.1.5 Mn2+ activator of isoform APY6 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 36989
-
 x * 36989, isoform APY6, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 48748
-
 x * 48748, isoform APY5, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 49765
-
 x * 49765, isoform APY4, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 50004
-
 x * 50004, isoform APY9, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 50032
-
 x * 50032, isoform APY8, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 50062
-
 x * 50062, isoform APY10, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 50077
-
 x * 50077, isoform APY7, calculated from amino acid sequence Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Solanum tuberosum 68% activity compared to ATP AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Solanum tuberosum 100% activity AMP + 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Solanum tuberosum
-
 variety Saturna
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5 isoform APY6, Superdex 75 gel filtration Solanum tuberosum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.6.1.5 isoform APY6 in 50 mM Tris-HCl pH 6.5 buffer containing 6 M guanidine hydrochloride and 10 mM 2-mercaptoethanol Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O 68% activity compared to ATP Solanum tuberosum AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O 100% activity Solanum tuberosum AMP + 2 phosphate
-
 ?
3.6.1.5 CDP + H2O 3.34% activity compared to ATP Solanum tuberosum CMP + phosphate
-
 ?
3.6.1.5 CTP + 2 H2O 12.5% activity compared to ATP Solanum tuberosum CMP + 2 phosphate
-
 ?
3.6.1.5 GDP + H2O 69.2% activity compared to ATP Solanum tuberosum GMP + phosphate
-
 ?
3.6.1.5 GTP + 2 H2O 84.6% activity compared to ATP Solanum tuberosum GMP + 2 phosphate
-
 ?
3.6.1.5 UDP + H2O 6.8% activity compared to ATP Solanum tuberosum UMP + phosphate
-
 ?
3.6.1.5 UTP + 2 H2O 28.2% activity compared to ATP Solanum tuberosum UMP + 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 ? x * 36989, isoform APY6, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 ? x * 48748, isoform APY5, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 ? x * 49765, isoform APY4, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 ? x * 50004, isoform APY9, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 ? x * 50032, isoform APY8, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 ? x * 50062, isoform APY10, calculated from amino acid sequence Solanum tuberosum
3.6.1.5 ? x * 50077, isoform APY7, calculated from amino acid sequence Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
3.6.1.5 APY10 isoform Solanum tuberosum
3.6.1.5 APY4 isoform Solanum tuberosum
3.6.1.5 APY5 isoform Solanum tuberosum
3.6.1.5 APY6 isoform Solanum tuberosum
3.6.1.5 APY7 isoform Solanum tuberosum
3.6.1.5 APY8 isoform Solanum tuberosum
3.6.1.5 APY9 isoform Solanum tuberosum
3.6.1.5 NTPDase
-
 Solanum tuberosum
3.6.1.5 nucleoside triphosphate diphosphohydrolase
-
 Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 6.5
-
 isoform APY6 Solanum tuberosum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.5 Solanum tuberosum isoform APY4, calculated from amino acid sequence
-
 6.6
3.6.1.5 Solanum tuberosum isoform APY7, calculated from amino acid sequence
-
 6.6
3.6.1.5 Solanum tuberosum isoform APY8, calculated from amino acid sequence
-
 6.6
3.6.1.5 Solanum tuberosum isoform APY9, calculated from amino acid sequence
-
 6.6
3.6.1.5 Solanum tuberosum isoform APY10, calculated from amino acid sequence
-
 7.2
3.6.1.5 Solanum tuberosum isoform APY5, calculated from amino acid sequence
-
 7.2
3.6.1.5 Solanum tuberosum isoform APY6, calculated from amino acid sequence
-
 8.4