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Literature summary extracted from

  • Brandt, W.; Manke, K.; Vogt, T.
    A catalytic triad - Lys-Asn-Asp - is essential for the catalysis of the methyl transfer in plant cation-dependent O-methyltransferases (2015), Phytochemistry, 113, 130-139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104
-
 Mesembryanthemum crystallinum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.104 D228A only residual enzyme activities are detectable Mesembryanthemum crystallinum
2.1.1.104 K157A mutation decreases Km but not the kcat value of eriodictyol, a single, unusual product is formed, namely the yellow fluorescing quercetagetin 5-O-methyl ether Mesembryanthemum crystallinum
2.1.1.104 N181A complete loss of activity with eriodictyol Mesembryanthemum crystallinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.00033
-
 quercetagetin wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.0014
-
 quercetagetin mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.003
-
 eriodictyol wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.0098
-
 quercetagetin mutant N181A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.0225
-
 eriodictyol mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.184
-
 eriodictyol mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.249
-
 quercetagetin mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Mesembryanthemum crystallinum Q6YI95
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 additional information a catalytic triad, consisting of Lys157-Asn181-Asp228 residues is required for complete methyl transfer in case of a cation-dependent phenylpropanoid and flavonoid O-methyl transferase. This triad appears essential for efficient methyl transfer to catechol-like hydroxyl group in phenolics. The triad is conserved among all characterized plant caffeoyl coenzyme A O-methyltransferase-like enzymes Mesembryanthemum crystallinum ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + eriodictyol
-
 Mesembryanthemum crystallinum S-adenosyl-L-homocysteine + homoeriodictyol single product ?
2.1.1.104 S-adenosyl-L-methionine + quercetagetin
-
 Mesembryanthemum crystallinum S-adenosyl-L-homocysteine + quercetagetin 6,3'-di-O-methylether
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.104 0.0016
-
 eriodictyol mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.0021
-
 quercetagetin mutant N181A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.0028
-
 eriodictyol mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.0116
-
 quercetagetin mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.078
-
 quercetagetin wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.1
-
 eriodictyol wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.106
-
 quercetagetin mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.104 0.0086
-
 eriodictyol mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.123
-
 eriodictyol mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.214
-
 quercetagetin mutant N181A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 0.426
-
 quercetagetin mutant K157A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 8.285
-
 quercetagetin mutant D228A, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 33.2
-
 eriodictyol wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum
2.1.1.104 237
-
 quercetagetin wild-type, pH 7.5, temperature not specified in the publication Mesembryanthemum crystallinum