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Literature summary extracted from

  • Haferkamp, P.
    Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus (2011), PH. D. Thesis Universität Duisburg-Essen, 2011, 0000.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 expression in Escherichia coli Saccharolobus solfataricus
2.7.1.40 expression in Escherichia coli Saccharolobus solfataricus
2.7.9.2 expression in Escherichia coli Saccharolobus solfataricus
4.2.1.11 expression in Escherichia coli Saccharolobus solfataricus
5.4.2.11 expression in Escherichia coli Saccharolobus solfataricus
5.4.2.12 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 ATP product inhibition. Inhibition cannot be reversed by presence of phophoenolpyruvate or ADP Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.14
-
NADP+ cosubstrate D-glucose, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 0.15
-
NADP+ cosubstrate D-glucose, 65°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 0.15
-
NADP+ cosubstrate D-glucose, 80°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 0.17
-
D-glucose cosubstrate NADP+, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 2.23
-
NAD+ cosubstrate D-glucose, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 3.3
-
NAD+ cosubstrate D-glucose, 80°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 4.59
-
D-glucose cosubstrate NAD+, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 5.59
-
NAD+ cosubstrate D-glucose, 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.069
-
ADP 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.093
-
phosphoenolpyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.113
-
ADP 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.115
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.137
-
ADP 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.164
-
ADP 50°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.225
-
phosphoenolpyruvate 50°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 0.26
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.46
-
pyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.47
-
ATP 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.54
-
pyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.61
-
ATP 70°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 0.06
-
2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 0.18
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 0.392
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 0.538
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 0.04
-
3-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 0.1
-
3-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 0.11
-
3-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 0.11
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 0.15
-
2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 0.26
-
2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 39000
-
x * 39000, SDS-PAGE, x * 39970, calculated Saccharolobus solfataricus
1.1.1.47 39970
-
x * 39000, SDS-PAGE, x * 39970, calculated Saccharolobus solfataricus
2.7.1.40 49800
-
x * 50000, SDS-PAGE, x * 49800, calculated Saccharolobus solfataricus
2.7.1.40 50000
-
x * 50000, SDS-PAGE, x * 49800, calculated Saccharolobus solfataricus
2.7.9.2 89300
-
x * 89000-92000, SDS-PAGE, x * 89300, calculated Saccharolobus solfataricus
4.2.1.11 46700
-
x * 48000-52000, SDS-PAGE, x * 46700, calculated Saccharolobus solfataricus
5.4.2.12 46800
-
x * 46000-48000, SDS-PAGE, x * 46800, calculated Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Saccharolobus solfataricus Q97U21
-
-
1.1.1.47 Saccharolobus solfataricus DSM 1617 Q97U21
-
-
2.7.1.40 Saccharolobus solfataricus Q97ZD7
-
-
2.7.1.40 Saccharolobus solfataricus DSM 1617 Q97ZD7
-
-
2.7.9.2 Saccharolobus solfataricus Q97V40
-
-
2.7.9.2 Saccharolobus solfataricus DSM 1617 Q97V40
-
-
4.2.1.11 Saccharolobus solfataricus Q97ZJ3
-
-
4.2.1.11 Saccharolobus solfataricus DSM 1617 Q97ZJ3
-
-
5.4.2.11 Saccharolobus solfataricus Q97WI1
-
-
5.4.2.11 Saccharolobus solfataricus DSM 1617 Q97WI1
-
-
5.4.2.12 Saccharolobus solfataricus Q980A0
-
-
5.4.2.12 Saccharolobus solfataricus DSM 1617 Q980A0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 D-galactose + NADP+ activity with substrate D-galactose in presence of cosubstrate NADP+ Saccharolobus solfataricus D-galactono-1,5-lactone + NADPH
-
?
1.1.1.47 D-galactose + NADP+ activity with substrate D-galactose in presence of cosubstrate NADP+ Saccharolobus solfataricus DSM 1617 D-galactono-1,5-lactone + NADPH
-
?
1.1.1.47 D-glucose + NAD+
-
Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 D-glucose + NAD+
-
Saccharolobus solfataricus DSM 1617 D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 D-xylose + NADP+ activity with substrate D-galactose in presence of cosubstrate NADP+ Saccharolobus solfataricus D-xylono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 D-xylose + NADP+ activity with substrate D-galactose in presence of cosubstrate NADP+ Saccharolobus solfataricus DSM 1617 D-xylono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 additional information no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol Saccharolobus solfataricus ?
-
?
1.1.1.47 additional information no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol Saccharolobus solfataricus DSM 1617 ?
-
?
2.7.1.40 ADP + phosphoenolpyruvate
-
Saccharolobus solfataricus ATP + pyruvate
-
r
2.7.1.40 ADP + phosphoenolpyruvate
-
Saccharolobus solfataricus DSM 1617 ATP + pyruvate
-
r
2.7.1.40 ATP + pyruvate
-
Saccharolobus solfataricus ADP + phosphoenolpyruvate
-
r
2.7.1.40 ATP + pyruvate
-
Saccharolobus solfataricus DSM 1617 ADP + phosphoenolpyruvate
-
r
2.7.9.2 ATP + pyruvate + H2O
-
Saccharolobus solfataricus AMP + phosphoenolpyruvate + phosphate
-
?
2.7.9.2 ATP + pyruvate + H2O
-
Saccharolobus solfataricus DSM 1617 AMP + phosphoenolpyruvate + phosphate
-
?
4.2.1.11 2-phospho-D-glycerate
-
Saccharolobus solfataricus phosphoenolpyruvate + H2O
-
r
4.2.1.11 2-phospho-D-glycerate
-
Saccharolobus solfataricus DSM 1617 phosphoenolpyruvate + H2O
-
r
4.2.1.11 phosphoenolpyruvate + H2O
-
Saccharolobus solfataricus 2-phospho-D-glycerate
-
r
4.2.1.11 phosphoenolpyruvate + H2O
-
Saccharolobus solfataricus DSM 1617 2-phospho-D-glycerate
-
r
5.4.2.12 2-phospho-D-glycerate
-
Saccharolobus solfataricus 3-phospho-D-glycerate
-
?
5.4.2.12 2-phospho-D-glycerate
-
Saccharolobus solfataricus DSM 1617 3-phospho-D-glycerate
-
?
5.4.2.12 3-phospho-D-glycerate
-
Saccharolobus solfataricus 2-phospho-D-glycerate
-
?
5.4.2.12 3-phospho-D-glycerate
-
Saccharolobus solfataricus DSM 1617 2-phospho-D-glycerate
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 ? x * 39000, SDS-PAGE, x * 39970, calculated Saccharolobus solfataricus
2.7.1.40 ? x * 50000, SDS-PAGE, x * 49800, calculated Saccharolobus solfataricus
2.7.9.2 ? x * 89000-92000, SDS-PAGE, x * 89300, calculated Saccharolobus solfataricus
4.2.1.11 ? x * 48000-52000, SDS-PAGE, x * 46700, calculated Saccharolobus solfataricus
5.4.2.12 ? x * 46000-48000, SDS-PAGE, x * 46800, calculated Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 glucose 1-dehydrogenase 3
-
Saccharolobus solfataricus
1.1.1.47 SSO3204
-
Saccharolobus solfataricus
2.7.1.40 SSO0981
-
Saccharolobus solfataricus
2.7.9.2 SSO2792
-
Saccharolobus solfataricus
4.2.1.11 ENO
-
Saccharolobus solfataricus
4.2.1.11 SSO0913
-
Saccharolobus solfataricus
5.4.2.11 dPGAM
-
Saccharolobus solfataricus
5.4.2.11 SSO2236
-
Saccharolobus solfataricus
5.4.2.12 apgM
-
Saccharolobus solfataricus
5.4.2.12 SSO0417
-
Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 19.34
-
D-glucose cosubstrate NADP+, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 26.74
-
NADP+ cosubstrate D-glucose, 65°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 28.52
-
NADP+ cosubstrate D-glucose, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 44.17
-
NADP+ cosubstrate D-glucose, 80°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 54.47
-
NAD+ cosubstrate D-glucose, 65°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 68.23
-
D-glucose cosubstrate NAD+, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 70.49
-
NAD+ cosubstrate D-glucose, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 105.4
-
NAD+ cosubstrate D-glucose, 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 39.67
-
phosphoenolpyruvate 50°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 40.06
-
ADP 50°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 50.12
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 52.85
-
ADP 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 58.4
-
phosphoenolpyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 58.78
-
ADP 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 73.04
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 80.29
-
ADP 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.25
-
ATP 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.326
-
ATP 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.41
-
pyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.48
-
pyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 1.51
-
3-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 2.04
-
2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 2.18
-
3-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 2.31
-
2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 3.01
-
3-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 4.01
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NADP+
-
Saccharolobus solfataricus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.40 0.8
-
65°C, pH not specified in the publication Saccharolobus solfataricus ATP
2.7.1.40 0.8
-
70°C, pH not specified in the publication Saccharolobus solfataricus ATP
2.7.1.40 0.8
-
80°C, pH not specified in the publication Saccharolobus solfataricus ATP

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.47 9.74
-
NAD+ cosubstrate D-glucose, 65°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 14.86
-
D-glucose cosubstrate NAD+, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 31.6
-
NAD+ cosubstrate D-glucose, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 31.95
-
NAD+ cosubstrate D-glucose, 80°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 115.2
-
D-glucose cosubstrate NADP+, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 178.3
-
NADP+ cosubstrate D-glucose, 65°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 203.7
-
NADP+ cosubstrate D-glucose, 70°C, pH not specified in the publication Saccharolobus solfataricus
1.1.1.47 294.5
-
NADP+ cosubstrate D-glucose, 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 137.3
-
phosphoenolpyruvate 50°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 244.3
-
ADP 50°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 280.9
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 385.8
-
ADP 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 435.8
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 628
-
phosphoenolpyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 710.5
-
ADP 80°C, pH not specified in the publication Saccharolobus solfataricus
2.7.1.40 851.9
-
ADP 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.53
-
ATP 70°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.53
-
ATP 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 0.75
-
pyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
2.7.9.2 1.04
-
pyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 58.44
-
phosphoenolpyruvate 65°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 91.88
-
phosphoenolpyruvate 70°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 110.8
-
phosphoenolpyruvate 80°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 831.5
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 909.5
-
2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
4.2.1.11 941.9
-
2-phospho-D-glycerate 88°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 7.84
-
2-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 15.4
-
2-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 19.81
-
3-phospho-D-glycerate 70°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 27.36
-
3-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 35.95
-
3-phospho-D-glycerate 65°C, pH not specified in the publication Saccharolobus solfataricus
5.4.2.12 36.45
-
2-phospho-D-glycerate 80°C, pH not specified in the publication Saccharolobus solfataricus