EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.47 | expression in Escherichia coli | Saccharolobus solfataricus |
2.7.1.40 | expression in Escherichia coli | Saccharolobus solfataricus |
2.7.9.2 | expression in Escherichia coli | Saccharolobus solfataricus |
4.2.1.11 | expression in Escherichia coli | Saccharolobus solfataricus |
5.4.2.11 | expression in Escherichia coli | Saccharolobus solfataricus |
5.4.2.12 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.40 | ATP | product inhibition. Inhibition cannot be reversed by presence of phophoenolpyruvate or ADP | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 0.14 | - |
NADP+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 0.15 | - |
NADP+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 0.15 | - |
NADP+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 0.17 | - |
D-glucose | cosubstrate NADP+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 2.23 | - |
NAD+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 3.3 | - |
NAD+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 4.59 | - |
D-glucose | cosubstrate NAD+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 5.59 | - |
NAD+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.069 | - |
ADP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.093 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.113 | - |
ADP | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.115 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.137 | - |
ADP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.164 | - |
ADP | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.225 | - |
phosphoenolpyruvate | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 0.26 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.46 | - |
pyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.47 | - |
ATP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.54 | - |
pyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.61 | - |
ATP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 0.06 | - |
2-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 0.18 | - |
2-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 0.392 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 0.538 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 0.04 | - |
3-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 0.1 | - |
3-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 0.11 | - |
3-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 0.11 | - |
2-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 0.15 | - |
2-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 0.26 | - |
2-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 39000 | - |
x * 39000, SDS-PAGE, x * 39970, calculated | Saccharolobus solfataricus |
1.1.1.47 | 39970 | - |
x * 39000, SDS-PAGE, x * 39970, calculated | Saccharolobus solfataricus |
2.7.1.40 | 49800 | - |
x * 50000, SDS-PAGE, x * 49800, calculated | Saccharolobus solfataricus |
2.7.1.40 | 50000 | - |
x * 50000, SDS-PAGE, x * 49800, calculated | Saccharolobus solfataricus |
2.7.9.2 | 89300 | - |
x * 89000-92000, SDS-PAGE, x * 89300, calculated | Saccharolobus solfataricus |
4.2.1.11 | 46700 | - |
x * 48000-52000, SDS-PAGE, x * 46700, calculated | Saccharolobus solfataricus |
5.4.2.12 | 46800 | - |
x * 46000-48000, SDS-PAGE, x * 46800, calculated | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.47 | Saccharolobus solfataricus | Q97U21 | - |
- |
1.1.1.47 | Saccharolobus solfataricus DSM 1617 | Q97U21 | - |
- |
2.7.1.40 | Saccharolobus solfataricus | Q97ZD7 | - |
- |
2.7.1.40 | Saccharolobus solfataricus DSM 1617 | Q97ZD7 | - |
- |
2.7.9.2 | Saccharolobus solfataricus | Q97V40 | - |
- |
2.7.9.2 | Saccharolobus solfataricus DSM 1617 | Q97V40 | - |
- |
4.2.1.11 | Saccharolobus solfataricus | Q97ZJ3 | - |
- |
4.2.1.11 | Saccharolobus solfataricus DSM 1617 | Q97ZJ3 | - |
- |
5.4.2.11 | Saccharolobus solfataricus | Q97WI1 | - |
- |
5.4.2.11 | Saccharolobus solfataricus DSM 1617 | Q97WI1 | - |
- |
5.4.2.12 | Saccharolobus solfataricus | Q980A0 | - |
- |
5.4.2.12 | Saccharolobus solfataricus DSM 1617 | Q980A0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | D-galactose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus | D-galactono-1,5-lactone + NADPH | - |
? | |
1.1.1.47 | D-galactose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus DSM 1617 | D-galactono-1,5-lactone + NADPH | - |
? | |
1.1.1.47 | D-glucose + NAD+ | - |
Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-glucose + NAD+ | - |
Saccharolobus solfataricus DSM 1617 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-xylose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.47 | D-xylose + NADP+ | activity with substrate D-galactose in presence of cosubstrate NADP+ | Saccharolobus solfataricus DSM 1617 | D-xylono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.47 | additional information | no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol | Saccharolobus solfataricus | ? | - |
? | |
1.1.1.47 | additional information | no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol | Saccharolobus solfataricus DSM 1617 | ? | - |
? | |
2.7.1.40 | ADP + phosphoenolpyruvate | - |
Saccharolobus solfataricus | ATP + pyruvate | - |
r | |
2.7.1.40 | ADP + phosphoenolpyruvate | - |
Saccharolobus solfataricus DSM 1617 | ATP + pyruvate | - |
r | |
2.7.1.40 | ATP + pyruvate | - |
Saccharolobus solfataricus | ADP + phosphoenolpyruvate | - |
r | |
2.7.1.40 | ATP + pyruvate | - |
Saccharolobus solfataricus DSM 1617 | ADP + phosphoenolpyruvate | - |
r | |
2.7.9.2 | ATP + pyruvate + H2O | - |
Saccharolobus solfataricus | AMP + phosphoenolpyruvate + phosphate | - |
? | |
2.7.9.2 | ATP + pyruvate + H2O | - |
Saccharolobus solfataricus DSM 1617 | AMP + phosphoenolpyruvate + phosphate | - |
? | |
4.2.1.11 | 2-phospho-D-glycerate | - |
Saccharolobus solfataricus | phosphoenolpyruvate + H2O | - |
r | |
4.2.1.11 | 2-phospho-D-glycerate | - |
Saccharolobus solfataricus DSM 1617 | phosphoenolpyruvate + H2O | - |
r | |
4.2.1.11 | phosphoenolpyruvate + H2O | - |
Saccharolobus solfataricus | 2-phospho-D-glycerate | - |
r | |
4.2.1.11 | phosphoenolpyruvate + H2O | - |
Saccharolobus solfataricus DSM 1617 | 2-phospho-D-glycerate | - |
r | |
5.4.2.12 | 2-phospho-D-glycerate | - |
Saccharolobus solfataricus | 3-phospho-D-glycerate | - |
? | |
5.4.2.12 | 2-phospho-D-glycerate | - |
Saccharolobus solfataricus DSM 1617 | 3-phospho-D-glycerate | - |
? | |
5.4.2.12 | 3-phospho-D-glycerate | - |
Saccharolobus solfataricus | 2-phospho-D-glycerate | - |
? | |
5.4.2.12 | 3-phospho-D-glycerate | - |
Saccharolobus solfataricus DSM 1617 | 2-phospho-D-glycerate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.47 | ? | x * 39000, SDS-PAGE, x * 39970, calculated | Saccharolobus solfataricus |
2.7.1.40 | ? | x * 50000, SDS-PAGE, x * 49800, calculated | Saccharolobus solfataricus |
2.7.9.2 | ? | x * 89000-92000, SDS-PAGE, x * 89300, calculated | Saccharolobus solfataricus |
4.2.1.11 | ? | x * 48000-52000, SDS-PAGE, x * 46700, calculated | Saccharolobus solfataricus |
5.4.2.12 | ? | x * 46000-48000, SDS-PAGE, x * 46800, calculated | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.47 | glucose 1-dehydrogenase 3 | - |
Saccharolobus solfataricus |
1.1.1.47 | SSO3204 | - |
Saccharolobus solfataricus |
2.7.1.40 | SSO0981 | - |
Saccharolobus solfataricus |
2.7.9.2 | SSO2792 | - |
Saccharolobus solfataricus |
4.2.1.11 | ENO | - |
Saccharolobus solfataricus |
4.2.1.11 | SSO0913 | - |
Saccharolobus solfataricus |
5.4.2.11 | dPGAM | - |
Saccharolobus solfataricus |
5.4.2.11 | SSO2236 | - |
Saccharolobus solfataricus |
5.4.2.12 | apgM | - |
Saccharolobus solfataricus |
5.4.2.12 | SSO0417 | - |
Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 19.34 | - |
D-glucose | cosubstrate NADP+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 26.74 | - |
NADP+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 28.52 | - |
NADP+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 44.17 | - |
NADP+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 54.47 | - |
NAD+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 68.23 | - |
D-glucose | cosubstrate NAD+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 70.49 | - |
NAD+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 105.4 | - |
NAD+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 39.67 | - |
phosphoenolpyruvate | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 40.06 | - |
ADP | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 50.12 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 52.85 | - |
ADP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 58.4 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 58.78 | - |
ADP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 73.04 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 80.29 | - |
ADP | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.25 | - |
ATP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.326 | - |
ATP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.41 | - |
pyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.48 | - |
pyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 1.51 | - |
3-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 2.04 | - |
2-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 2.18 | - |
3-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 2.31 | - |
2-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 3.01 | - |
3-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 4.01 | - |
2-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | NADP+ | - |
Saccharolobus solfataricus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.1.40 | 0.8 | - |
65°C, pH not specified in the publication | Saccharolobus solfataricus | ATP | |
2.7.1.40 | 0.8 | - |
70°C, pH not specified in the publication | Saccharolobus solfataricus | ATP | |
2.7.1.40 | 0.8 | - |
80°C, pH not specified in the publication | Saccharolobus solfataricus | ATP |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 9.74 | - |
NAD+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 14.86 | - |
D-glucose | cosubstrate NAD+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 31.6 | - |
NAD+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 31.95 | - |
NAD+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 115.2 | - |
D-glucose | cosubstrate NADP+, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 178.3 | - |
NADP+ | cosubstrate D-glucose, 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 203.7 | - |
NADP+ | cosubstrate D-glucose, 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
1.1.1.47 | 294.5 | - |
NADP+ | cosubstrate D-glucose, 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 137.3 | - |
phosphoenolpyruvate | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 244.3 | - |
ADP | 50°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 280.9 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 385.8 | - |
ADP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 435.8 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 628 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 710.5 | - |
ADP | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.1.40 | 851.9 | - |
ADP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.53 | - |
ATP | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.53 | - |
ATP | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 0.75 | - |
pyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
2.7.9.2 | 1.04 | - |
pyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 58.44 | - |
phosphoenolpyruvate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 91.88 | - |
phosphoenolpyruvate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 110.8 | - |
phosphoenolpyruvate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 831.5 | - |
2-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 909.5 | - |
2-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
4.2.1.11 | 941.9 | - |
2-phospho-D-glycerate | 88°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 7.84 | - |
2-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 15.4 | - |
2-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 19.81 | - |
3-phospho-D-glycerate | 70°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 27.36 | - |
3-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 35.95 | - |
3-phospho-D-glycerate | 65°C, pH not specified in the publication | Saccharolobus solfataricus | |
5.4.2.12 | 36.45 | - |
2-phospho-D-glycerate | 80°C, pH not specified in the publication | Saccharolobus solfataricus |