BRENDA - Enzyme Database

Biochemical studies of enzymes involved in glycolysis of the thermoacidophilic crenarchaeon Sulfolobus solfataricus

Haferkamp, P.; PH. D. Thesis Universität Duisburg-Essen 2011, 0000 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.47
expression in Escherichia coli
Saccharolobus solfataricus
2.7.1.40
expression in Escherichia coli
Saccharolobus solfataricus
2.7.9.2
expression in Escherichia coli
Saccharolobus solfataricus
4.2.1.11
expression in Escherichia coli
Saccharolobus solfataricus
5.4.2.11
expression in Escherichia coli
Saccharolobus solfataricus
5.4.2.12
expression in Escherichia coli
Saccharolobus solfataricus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
ATP
product inhibition. Inhibition cannot be reversed by presence of phophoenolpyruvate or ADP
Saccharolobus solfataricus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.47
0.14
-
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
0.15
-
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication; cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
0.17
-
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
2.23
-
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
3.3
-
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
4.59
-
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
5.59
-
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.069
-
ADP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.093
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.113
-
ADP
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.115
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.137
-
ADP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.164
-
ADP
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.225
-
phosphoenolpyruvate
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.26
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.46
-
pyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.47
-
ATP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.54
-
pyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.61
-
ATP
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.06
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.18
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.392
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.538
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.04
-
3-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.1
-
3-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.11
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.11
-
3-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.15
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.26
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.47
39000
-
x * 39000, SDS-PAGE, x * 39970, calculated
Saccharolobus solfataricus
1.1.1.47
39970
-
x * 39000, SDS-PAGE, x * 39970, calculated
Saccharolobus solfataricus
2.7.1.40
49800
-
x * 50000, SDS-PAGE, x * 49800, calculated
Saccharolobus solfataricus
2.7.1.40
50000
-
x * 50000, SDS-PAGE, x * 49800, calculated
Saccharolobus solfataricus
2.7.9.2
89300
-
x * 89000-92000, SDS-PAGE, x * 89300, calculated
Saccharolobus solfataricus
4.2.1.11
46700
-
x * 48000-52000, SDS-PAGE, x * 46700, calculated
Saccharolobus solfataricus
5.4.2.12
46800
-
x * 46000-48000, SDS-PAGE, x * 46800, calculated
Saccharolobus solfataricus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.47
Saccharolobus solfataricus
Q97U21
-
-
1.1.1.47
Saccharolobus solfataricus DSM 1617
Q97U21
-
-
2.7.1.40
Saccharolobus solfataricus
Q97ZD7
-
-
2.7.1.40
Saccharolobus solfataricus DSM 1617
Q97ZD7
-
-
2.7.9.2
Saccharolobus solfataricus
Q97V40
-
-
2.7.9.2
Saccharolobus solfataricus DSM 1617
Q97V40
-
-
4.2.1.11
Saccharolobus solfataricus
Q97ZJ3
-
-
4.2.1.11
Saccharolobus solfataricus DSM 1617
Q97ZJ3
-
-
5.4.2.11
Saccharolobus solfataricus
Q97WI1
-
-
5.4.2.11
Saccharolobus solfataricus DSM 1617
Q97WI1
-
-
5.4.2.12
Saccharolobus solfataricus
Q980A0
-
-
5.4.2.12
Saccharolobus solfataricus DSM 1617
Q980A0
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.47
D-galactose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus
D-galactono-1,5-lactone + NADPH
-
-
-
?
1.1.1.47
D-galactose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus DSM 1617
D-galactono-1,5-lactone + NADPH
-
-
-
?
1.1.1.47
D-glucose + NAD+
-
734842
Saccharolobus solfataricus
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
1.1.1.47
D-glucose + NAD+
-
734842
Saccharolobus solfataricus DSM 1617
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
1.1.1.47
D-xylose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
1.1.1.47
D-xylose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus DSM 1617
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
1.1.1.47
additional information
no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol
734842
Saccharolobus solfataricus
?
-
-
-
-
1.1.1.47
additional information
no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol
734842
Saccharolobus solfataricus DSM 1617
?
-
-
-
-
2.7.1.40
ADP + phosphoenolpyruvate
-
734842
Saccharolobus solfataricus
ATP + pyruvate
-
-
-
r
2.7.1.40
ADP + phosphoenolpyruvate
-
734842
Saccharolobus solfataricus DSM 1617
ATP + pyruvate
-
-
-
r
2.7.1.40
ATP + pyruvate
-
734842
Saccharolobus solfataricus
ADP + phosphoenolpyruvate
-
-
-
r
2.7.1.40
ATP + pyruvate
-
734842
Saccharolobus solfataricus DSM 1617
ADP + phosphoenolpyruvate
-
-
-
r
2.7.9.2
ATP + pyruvate + H2O
-
734842
Saccharolobus solfataricus
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
2.7.9.2
ATP + pyruvate + H2O
-
734842
Saccharolobus solfataricus DSM 1617
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus DSM 1617
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
734842
Saccharolobus solfataricus
2-phospho-D-glycerate
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
734842
Saccharolobus solfataricus DSM 1617
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus
3-phospho-D-glycerate
-
-
-
?
5.4.2.12
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus DSM 1617
3-phospho-D-glycerate
-
-
-
?
5.4.2.12
3-phospho-D-glycerate
-
734842
Saccharolobus solfataricus
2-phospho-D-glycerate
-
-
-
?
5.4.2.12
3-phospho-D-glycerate
-
734842
Saccharolobus solfataricus DSM 1617
2-phospho-D-glycerate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.47
?
x * 39000, SDS-PAGE, x * 39970, calculated
Saccharolobus solfataricus
2.7.1.40
?
x * 50000, SDS-PAGE, x * 49800, calculated
Saccharolobus solfataricus
2.7.9.2
?
x * 89000-92000, SDS-PAGE, x * 89300, calculated
Saccharolobus solfataricus
4.2.1.11
?
x * 48000-52000, SDS-PAGE, x * 46700, calculated
Saccharolobus solfataricus
5.4.2.12
?
x * 46000-48000, SDS-PAGE, x * 46800, calculated
Saccharolobus solfataricus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.47
19.34
-
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
26.74
-
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
28.52
-
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
44.17
-
NADP+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
54.47
-
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
68.23
-
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
70.49
-
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
105.4
-
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
39.67
-
phosphoenolpyruvate
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
40.06
-
ADP
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
50.12
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
52.85
-
ADP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
58.4
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
58.78
-
ADP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
73.04
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
80.29
-
ADP
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.25
-
ATP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.326
-
ATP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.41
-
pyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.48
-
pyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
1.51
-
3-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
2.04
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
2.18
-
3-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
2.31
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
3.01
-
3-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
4.01
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.47
NADP+
-
Saccharolobus solfataricus
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
0.8
-
65°C, pH not specified in the publication; 70°C, pH not specified in the publication; 80°C, pH not specified in the publication
Saccharolobus solfataricus
ATP
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.47
expression in Escherichia coli
Saccharolobus solfataricus
2.7.1.40
expression in Escherichia coli
Saccharolobus solfataricus
2.7.9.2
expression in Escherichia coli
Saccharolobus solfataricus
4.2.1.11
expression in Escherichia coli
Saccharolobus solfataricus
5.4.2.11
expression in Escherichia coli
Saccharolobus solfataricus
5.4.2.12
expression in Escherichia coli
Saccharolobus solfataricus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.47
NADP+
-
Saccharolobus solfataricus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
0.8
-
65°C, pH not specified in the publication; 70°C, pH not specified in the publication; 80°C, pH not specified in the publication
Saccharolobus solfataricus
ATP
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
ATP
product inhibition. Inhibition cannot be reversed by presence of phophoenolpyruvate or ADP
Saccharolobus solfataricus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.47
0.14
-
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
0.15
-
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication; cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
0.17
-
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
2.23
-
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
3.3
-
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
4.59
-
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
5.59
-
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.069
-
ADP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.093
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.113
-
ADP
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.115
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.137
-
ADP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.164
-
ADP
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.225
-
phosphoenolpyruvate
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
0.26
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.46
-
pyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.47
-
ATP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.54
-
pyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.61
-
ATP
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.06
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.18
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.392
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
0.538
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.04
-
3-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.1
-
3-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.11
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.11
-
3-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.15
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
0.26
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.47
39000
-
x * 39000, SDS-PAGE, x * 39970, calculated
Saccharolobus solfataricus
1.1.1.47
39970
-
x * 39000, SDS-PAGE, x * 39970, calculated
Saccharolobus solfataricus
2.7.1.40
49800
-
x * 50000, SDS-PAGE, x * 49800, calculated
Saccharolobus solfataricus
2.7.1.40
50000
-
x * 50000, SDS-PAGE, x * 49800, calculated
Saccharolobus solfataricus
2.7.9.2
89300
-
x * 89000-92000, SDS-PAGE, x * 89300, calculated
Saccharolobus solfataricus
4.2.1.11
46700
-
x * 48000-52000, SDS-PAGE, x * 46700, calculated
Saccharolobus solfataricus
5.4.2.12
46800
-
x * 46000-48000, SDS-PAGE, x * 46800, calculated
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.47
D-galactose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus
D-galactono-1,5-lactone + NADPH
-
-
-
?
1.1.1.47
D-galactose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus DSM 1617
D-galactono-1,5-lactone + NADPH
-
-
-
?
1.1.1.47
D-glucose + NAD+
-
734842
Saccharolobus solfataricus
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
1.1.1.47
D-glucose + NAD+
-
734842
Saccharolobus solfataricus DSM 1617
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
1.1.1.47
D-xylose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
1.1.1.47
D-xylose + NADP+
activity with substrate D-galactose in presence of cosubstrate NADP+
734842
Saccharolobus solfataricus DSM 1617
D-xylono-1,5-lactone + NADPH + H+
-
-
-
?
1.1.1.47
additional information
no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol
734842
Saccharolobus solfataricus
?
-
-
-
-
1.1.1.47
additional information
no substrates: D-allose, D-mannose, L-arabinose, D-ribose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose, ethanol
734842
Saccharolobus solfataricus DSM 1617
?
-
-
-
-
2.7.1.40
ADP + phosphoenolpyruvate
-
734842
Saccharolobus solfataricus
ATP + pyruvate
-
-
-
r
2.7.1.40
ADP + phosphoenolpyruvate
-
734842
Saccharolobus solfataricus DSM 1617
ATP + pyruvate
-
-
-
r
2.7.1.40
ATP + pyruvate
-
734842
Saccharolobus solfataricus
ADP + phosphoenolpyruvate
-
-
-
r
2.7.1.40
ATP + pyruvate
-
734842
Saccharolobus solfataricus DSM 1617
ADP + phosphoenolpyruvate
-
-
-
r
2.7.9.2
ATP + pyruvate + H2O
-
734842
Saccharolobus solfataricus
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
2.7.9.2
ATP + pyruvate + H2O
-
734842
Saccharolobus solfataricus DSM 1617
AMP + phosphoenolpyruvate + phosphate
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus DSM 1617
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
734842
Saccharolobus solfataricus
2-phospho-D-glycerate
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
734842
Saccharolobus solfataricus DSM 1617
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus
3-phospho-D-glycerate
-
-
-
?
5.4.2.12
2-phospho-D-glycerate
-
734842
Saccharolobus solfataricus DSM 1617
3-phospho-D-glycerate
-
-
-
?
5.4.2.12
3-phospho-D-glycerate
-
734842
Saccharolobus solfataricus
2-phospho-D-glycerate
-
-
-
?
5.4.2.12
3-phospho-D-glycerate
-
734842
Saccharolobus solfataricus DSM 1617
2-phospho-D-glycerate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.47
?
x * 39000, SDS-PAGE, x * 39970, calculated
Saccharolobus solfataricus
2.7.1.40
?
x * 50000, SDS-PAGE, x * 49800, calculated
Saccharolobus solfataricus
2.7.9.2
?
x * 89000-92000, SDS-PAGE, x * 89300, calculated
Saccharolobus solfataricus
4.2.1.11
?
x * 48000-52000, SDS-PAGE, x * 46700, calculated
Saccharolobus solfataricus
5.4.2.12
?
x * 46000-48000, SDS-PAGE, x * 46800, calculated
Saccharolobus solfataricus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.47
19.34
-
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
26.74
-
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
28.52
-
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
44.17
-
NADP+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
54.47
-
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
68.23
-
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
70.49
-
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
105.4
-
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
39.67
-
phosphoenolpyruvate
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
40.06
-
ADP
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
50.12
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
52.85
-
ADP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
58.4
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
58.78
-
ADP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
73.04
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
80.29
-
ADP
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.25
-
ATP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.326
-
ATP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.41
-
pyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.48
-
pyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
1.51
-
3-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
2.04
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
2.18
-
3-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
2.31
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
3.01
-
3-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
4.01
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.47
9.74
-
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
14.86
-
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
31.6
-
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
31.95
-
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
115.2
-
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
178.3
-
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
203.7
-
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
294.5
-
NADP+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
137.3
-
phosphoenolpyruvate
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
244.3
-
ADP
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
280.9
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
385.8
-
ADP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
435.8
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
628
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
710.5
-
ADP
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
851.9
-
ADP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.53
-
ATP
65°C, pH not specified in the publication; 70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.75
-
pyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
1.04
-
pyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
58.44
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
91.88
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
110.8
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
831.5
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
909.5
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
941.9
-
2-phospho-D-glycerate
88°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
7.84
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
15.4
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
19.81
-
3-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
27.36
-
3-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
35.95
-
3-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
36.45
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.47
9.74
-
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
14.86
-
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
31.6
-
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
31.95
-
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
115.2
-
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
178.3
-
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
203.7
-
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
Saccharolobus solfataricus
1.1.1.47
294.5
-
NADP+
cosubstrate D-glucose, 80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
137.3
-
phosphoenolpyruvate
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
244.3
-
ADP
50°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
280.9
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
385.8
-
ADP
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
435.8
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
628
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
710.5
-
ADP
80°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.1.40
851.9
-
ADP
70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.53
-
ATP
65°C, pH not specified in the publication; 70°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
0.75
-
pyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
2.7.9.2
1.04
-
pyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
58.44
-
phosphoenolpyruvate
65°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
91.88
-
phosphoenolpyruvate
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
110.8
-
phosphoenolpyruvate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
831.5
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
909.5
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
4.2.1.11
941.9
-
2-phospho-D-glycerate
88°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
7.84
-
2-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
15.4
-
2-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
19.81
-
3-phospho-D-glycerate
70°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
27.36
-
3-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
35.95
-
3-phospho-D-glycerate
65°C, pH not specified in the publication
Saccharolobus solfataricus
5.4.2.12
36.45
-
2-phospho-D-glycerate
80°C, pH not specified in the publication
Saccharolobus solfataricus