Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Horton, J.; Zhang, X.; Blumenthal, R.; Cheng, X.
    Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: Potential implications for methylation-independent transcriptional repression (2015), Nucleic Acids Res., 43, 4296-4308.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli Hms174(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.72 in complex with non-cognate DNA, lacking any GATC sequences, hanging drop vapor diffusion method, using 5-40% (w/v) PEG 200 from and 100 mM MES or HEPES (pH 6.4-7.0) Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli
-
 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli P0AEE8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 Ni2+ affinity column chromatography, UnoS column chromatography, and S75 Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information relative to GATC, three near-cognate substrates that carry a base-pair substitution at the first position are still methylated by the enzyme, although at rates reduced by 100fold (AATC) or 1000fold (CATC) Escherichia coli ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 Dam
-
 Escherichia coli
2.1.1.72 DNA adenine methyltransferase
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.72 metabolism the enzyme, in addition to being responsible for GATC methylation, can also function as a methylation-independent transcriptional repressor Escherichia coli