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Literature summary extracted from

  • Balakrishnan, R.; Oman, K.; Shoji, S.; Bundschuh, R.; Fredrick, K.
    The conserved GTPase LepA contributes mainly to translation initiation in Escherichia coli (2014), Nucleic Acids Res., 42, 13370-13383.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.3 H81A site-directed mutagenesis, impaired enzyme mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.3 GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O Escherichia coli BW25113
-
 GDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Escherichia coli
-
 gene lepA
-
3.6.5.3 Escherichia coli BW25113
-
 gene lepA
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.5.3 additional information growth rates of wild-type and mutant strains, overview Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O
-
 Escherichia coli BW25113 GDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.5.3 LepA
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
3.6.5.3 evolution LepA is a paralogue of elongation factor G found in all bacteria Escherichia coli
3.6.5.3 malfunction in strains DEltadksA, DELTAmolR1, DELTArsgA, DELTAtatB, DELTAtonB, DELTAtolR, DELTAubiF, DELTAubiG or DELTAubiH the deletion of lepA confers a synthetic growth phenotype. The strains are compromised for gene regulation, ribosome assembly, transport and/or respiration. Loss of LepA alters the average ribosome density for hundreds of mRNA coding regions in the cell, substantially reducing average ribosome density in many cases, but only subtle and codon-specific changes in ribosome distribution along mRNA are seen. Global perturbation of gene expression in the DELTAlepA mutant likely explains most of its phenotypes. LepA variants lacking the active-site histidine or unique C-terminal domain fail to complement the synthetic phenotypes Escherichia coli
3.6.5.3 additional information histidine 81 is critical for GTPase activity, both the C-terminal domain and the GTPase activity of LepA are critical for its function in vivo Escherichia coli
3.6.5.3 physiological function LepA contributes mainly to the initiation phase of translation. The effect of LepA on average ribosome density is related to the sequence of the Shine-Dalgarno region. But the enzyme does not generally influence polypeptide chain elongation rate Escherichia coli