Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Parker, J.L.; Newstead, S.
    Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1 (2014), Nature, 507, 68-72.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.4 expressed in Saccharomyces cerevisiae Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.3.2.4 apo and nitrate-bound enzyme, hanging drop vapor diffusion method, using 22% (w/v) PEG 400, 0.05 M sodium citrate pH 4.5, 0.07 M sodium chloride and 1.5% (w/v) PEG 600 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.4 H356A the mutation results in complete loss of nitrate binding ability Arabidopsis thaliana
7.3.2.4 T101D the variant shows increased nitrate uptake of approximately 2.8fold compared to the wild type enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.4 ATP + H2O + nitrate/out Arabidopsis thaliana
-
 ADP + phosphate + nitrate/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.4 Arabidopsis thaliana Q05085
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.3.2.4
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.4 ATP + H2O + nitrate/out
-
 Arabidopsis thaliana ADP + phosphate + nitrate/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.4 nitrate transporter
-
 Arabidopsis thaliana
7.3.2.4 NPF6.3
-
 Arabidopsis thaliana
7.3.2.4 NRT1.1
-
 Arabidopsis thaliana