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Literature summary extracted from

  • Ion, B.F.; Kazim, E.; Gauld, J.W.
    A multi-scale computational study on the mechanism of Streptococcus pneumoniae nicotinamidase (SpNic) (2014), Molecules, 19, 15735-15753.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.19 drug development while nicotinamidases are widespread in nature they are not occuring within mammals and present a potential drug target. The enzymes can be used in the activation of tuberculosis prodrugs such as pyrazinamide Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Zn2+ zinc-dependent enzyme Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Streptococcus pneumoniae
-
 nicotinate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Streptococcus pneumoniae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.19 nicotinamide + H2O = nicotinate + NH3 catalytic mechanism in two stages: (i) formation of a tetrahedral thioester enzyme-substrate intermediate (with the sulfur of Cys136 nucleophilically attacks the nicotinamidase substrate's carbonyl carbon while concomitantly the Cys136-SH thiol proton is transferred onto the substrate's amide group nitrogen) and (ii) hydrolysis of the thioester bond to give the products, overview. The catalytic triad comprises Lys103, Asp9, and Cys136, substrate binding and roles of active site residues of the enzyme from Streptococcus pneumoniae. The active site Lys103 likely plays a role in stabilizing the thiolate of Cys136 during the reaction Streptococcus pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
 Streptococcus pneumoniae nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 Streptococcus pneumoniae pyrazinoic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 NIC
-
 Streptococcus pneumoniae
3.5.1.19 nicotinamidase
-
 Streptococcus pneumoniae
3.5.1.19 SpNic
-
 Streptococcus pneumoniae

General Information

EC Number General Information Comment Organism
3.5.1.19 evolution nicotinamidases are a family of peptide hydrolases that generally contain a Zn2+ ion Streptococcus pneumoniae
3.5.1.19 metabolism nicotinamidase is a key enzyme in NAD+ metabolism Streptococcus pneumoniae
3.5.1.19 additional information catalytic mechanism, substrate binding and roles of active site residues, quantum mechanics/molecular mechanics methods and modeling, overview. The polar protein environment has a significant effect in stabilizing reaction intermediates and in particular transition states Streptococcus pneumoniae