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Literature summary extracted from

  • Galizzi, M.; Bustamante, J.M.; Fang, J.; Miranda, K.; Soares Medeiros, L.C.; Tarleton, R.L.; Docampo, R.
    Evidence for the role of vacuolar soluble pyrophosphatase and inorganic polyphosphate in Trypanosoma cruzi persistence (2013), Mol. Microbiol., 90, 699-715.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expressed in Escherichia coli BL21(DE3) cells as a fusion protein with a C-terminal polyhistidine tag Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.008
-
diphosphate with 3 mM Mg2+, at pH 8.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.0167
-
Triphosphate with 3 mM Co2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.0174
-
polyphosphate 75 with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.1554
-
guanosine 5'-tetraphosphate with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 acidocalcisome
-
Trypanosoma cruzi 20022
-
3.6.1.1 cytosol
-
Trypanosoma cruzi 5829
-
3.6.1.1 soluble
-
Trypanosoma cruzi
-
-
3.6.1.1 vacuole
-
Trypanosoma cruzi 5773
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Co2+ preferred cofactor for hydrolysis of triphosphate Trypanosoma cruzi
3.6.1.1 Mg2+ preferred cofactor for the hydrolysis of diphosphate, highest stimulation of activity at about 0.5 mM Trypanosoma cruzi
3.6.1.1 Zn2+ preferred cofactor for hydrolysis of polyphosphate 75 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 45000
-
x * 45000, His-tagged enzyme, SDS-PAGE Trypanosoma cruzi
3.6.1.1 47900
-
x * 47900, calculated from amino acid sequence Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Trypanosoma cruzi
-
2 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Trypanosoma cruzi
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.1 epimastigote
-
Trypanosoma cruzi
-
3.6.1.1 promastigote
-
Trypanosoma cruzi
-
3.6.1.1 trypomastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
Trypanosoma cruzi 2 phosphate
-
?
3.6.1.1 guanosine 5'-tetraphosphate + H2O
-
Trypanosoma cruzi ?
-
?
3.6.1.1 polyphosphate 75 + H2O
-
Trypanosoma cruzi ?
-
?
3.6.1.1 triphosphate + H2O
-
Trypanosoma cruzi ?
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 ? x * 45000, His-tagged enzyme, SDS-PAGE Trypanosoma cruzi
3.6.1.1 ? x * 47900, calculated from amino acid sequence Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 inorganic pyrophosphatase
-
Trypanosoma cruzi
3.6.1.1 PPase
-
Trypanosoma cruzi
3.6.1.1 VSP
-
Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.1 30
-
for the hydrolysis of diphosphate Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 0.000165
-
polyphosphate 75 with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.0022
-
guanosine 5'-tetraphosphate with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.13
-
Triphosphate with 3 mM Co2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.22
-
diphosphate with 3 mM Mg2+, at pH 8.5 and 30°C Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.1 6.5
-
for the hydrolysis of triphosphate or polyphosphate 75 Trypanosoma cruzi
3.6.1.1 8.5
-
for the hydrolysis of diphosphate Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.1 Trypanosoma cruzi calculated from amino acid sequence
-
6

General Information

EC Number General Information Comment Organism
3.6.1.1 physiological function enzyme-overexpressing parasites show a significant growth defect in fibroblasts, less responsiveness to hyperosmotic stress, and reduced persistence in tissues of mice Trypanosoma cruzi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.1 0.00141
-
guanosine 5'-tetraphosphate with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 0.00948
-
polyphosphate 75 with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 7.8
-
Triphosphate with 3 mM Co2+, at pH 6.5 and 30°C Trypanosoma cruzi
3.6.1.1 27
-
diphosphate with 3 mM Mg2+, at pH 8.5 and 30°C Trypanosoma cruzi