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Literature summary extracted from

  • McNally, K.; Berg, E.; Cortes, D.B.; Hernandez, V.; Mains, P.E.; McNally, F.J.
    Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro (2014), Mol. Biol. Cell, 25, 1037-1049.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.1 recombinant expression of untagged short isoform of MEI-1 subunit, coexpression with MBP-TEV-tagged MEI-2 subunit, in Escherichia coli Caenorhabditis elegans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.1 cytoplasm
-
 Caenorhabditis elegans 5737
-
5.6.1.1 microtubule
-
 Caenorhabditis elegans 5874
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.1 Mg2+ required Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.1 ATP + H2O Caenorhabditis elegans
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.1 Caenorhabditis elegans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.1 recombinant untagged short isoform of MEI-1 subunit and MBP-TEV-tagged MEI-2 subunit from Escherichia coli by amylose affinity chromatography, eluted with TEV protease Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.1 ATP + H2O
-
 Caenorhabditis elegans ADP + phosphate
-
 ?
5.6.1.1 additional information the enzyme has multiple effects on microtubules in vitro. Purified MEI-1/MEI-2 complexes preferentially sever at intersections be­tween two microtubules and directly bundle microtubules in vitro. These activities promote parallel/antiparallel microtubule organization in meiotic spindles, MEI-1/MEI-2 complexes bundle microtubules and preferentially sever at intersections between two microtubules in vitro Caenorhabditis elegans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.1 katanin
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
5.6.1.1 malfunction rapid inactivation of katanin results in complete loss of ASPM-1 positive spindle poles. Strong loss-of-function mutants assemble apolar intersecting microtubule arrays, whereas weaker mutants assemble bipolar meiotic spindles that are longer than wild-type. Microtubule disassembly in enzyme point mutants, overview. Progressive loss of katanin leads to a progressive loss in the organization of pole-to-pole microtubule bundles, phenotypes Caenorhabditis elegans
5.6.1.1 physiological function katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo. Assembly of Caenorhabditis elegans female meiotic spindles requires both MEI-1 and MEI-2 subunits of the microtubule-severing ATPase katanin. A linear relationship between in vitro microtubule disassembly rates and in vivo spindle length exists Caenorhabditis elegans