Literature summary extracted from

Soo, V.W.; McAnulty, M.J.; Tripathi, A.; Zhu, F.; Zhang, L.; Hatzakis, E.; Smith, P.B.; Agrawal, S.; Nazem-Bokaee, H.; Gopalakrishnan, S.; Salis, H.M.; Ferry, J.G.; Maranas, C.D.; Patterson, A.D.; Wood, T.K.
Reversing methanogenesis to capture methane for liquid biofuel precursors (2016), Microb. Cell Fact., 15, 11.

Application

EC Number	Application	Comment	Organism
2.8.4.1	environmental protection	expression of methyl-coenzyme M reductase from an unculturable organism in Methanosarcina acetivorans to effectively run methanogenesis in reverse. Methanosarcina acetivorans cells heterologously producing methyl-coenzyme M reductase consume up to 9% of methane (corresponding to 109 micromol of methane) after 6 weeks of anaerobic growth on methane and utilize 10 mM FeCl3 as an electron acceptor. When incubated on methane for 5 days, high-densities of cells consume 15% methane (corresponding to 143 micromol of methane), and produce 10.3 mM acetate (corresponding to 52 micromol of acetate)	uncultured bacterium
2.8.4.1	synthesis	expression of methyl-coenzyme M reductase from an unculturable organism in Methanosarcina acetivorans to effectively run methanogenesis in reverse. Methanosarcina acetivorans cells heterologously producing methyl-coenzyme M reductase consume up to 9% of methane (corresponding to 109 ± 12 micromol of methane) after 6 weeks of anaerobic growth on methane and utilize 10 mM FeCl3 as an electron acceptor. When incubated on methane for 5 days, high-densities of cells consume 15% methane (corresponding to 143 ± 16 micromol of methane), and produce 10.3 mM acetate (corresponding to 52 ± 4 µmol of acetate)	uncultured bacterium

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.8.4.1	-	uncultured bacterium

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.4.1	uncultured bacterium	-	i.e. anaerobic methanotrophic archaeal population 1, ANME-1	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.8.4.1	CoM-S-S-CoB + methane	-	uncultured bacterium	methyl-CoM + CoB	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.8.4.1	methyl-coenzyme M reductase	-	uncultured bacterium

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Release 2023.1 (January 2023)