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Literature summary extracted from

  • Gajadeera, C.S.; Zhang, X.; Wei, Y.; Tsodikov, O.V.
    Structure of inorganic pyrophosphatase from Staphylococcus aureus reveals conformational flexibility of the active site (2015), J. Struct. Biol., 189, 81-86.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.1 isoform PpaC in complex with Mn2+, hanging drop vapor diffusion method, using 1.32 M ammonium citrate tribasic Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.1 R295A inactive Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.1 cytoplasm
-
 Staphylococcus aureus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Mn2+ dependent on, the active site contains two catalytic Mn2+ binding sites Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 34000
-
 2 * 34000, SDS-PAGE Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.1 diphosphate + H2O Staphylococcus aureus
-
 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O Staphylococcus aureus Mu50
-
 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Staphylococcus aureus P65752
-
-
3.6.1.1 Staphylococcus aureus Mu50 P65752
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 Ni-IMAC HisTrap column chromatography and S-200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Staphylococcus aureus 2 phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Staphylococcus aureus Mu50 2 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 homodimer 2 * 34000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 inorganic pyrophosphatase
-
 Staphylococcus aureus
3.6.1.1 PpaC
-
 Staphylococcus aureus
3.6.1.1 PPIase
-
 Staphylococcus aureus