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Literature summary extracted from

  • Byeon, Y.; Lee, H.J.; Lee, H.Y.; Back, K.
    Cloning and functional characterization of the Arabidopsis N-acetylserotonin O-methyltransferase responsible for melatonin synthesis (2015), J. Pineal Res., 60, 65-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.4 gene cloning, DNA and amino acid sequence determination and analysis, the At4g35160 gene exhibits the highest sequence identity (31%) to the rice ASMT gene, followed by the At1g76790 gene with 29% sequence identity, phylogenetic analysis. The recombinant proteins are recombinantly expressed from the two Arabidopsis genes. The At4g35160 recombinant protein exhibits ASMT enzyme activity, but the At1g76790 recombinant protein does not. Recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.4 additional information ectopic overexpression of the enzyme in rice results in increased ASMT enzyme activity and melatonin production Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.4 additional information no inhibition by caffeic acid Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.4 0.456
-
 N-acetylserotonin pH 7.8, 37°C Arabidopsis thaliana
2.1.1.4 1.036
-
 serotonin pH 8.8, 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.4 cytoplasm
-
 Arabidopsis thaliana 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.4 additional information Arabidopsis thaliana the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis ?
-
 ?
2.1.1.4 additional information Arabidopsis thaliana Col-0 the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis ?
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin Arabidopsis thaliana Col-0
-
 S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.4 S-adenosyl-L-methionine + serotonin Arabidopsis thaliana enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?
2.1.1.4 S-adenosyl-L-methionine + serotonin Arabidopsis thaliana Col-0 enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.4 Arabidopsis thaliana Q9T003 gene At4g35160 or AtASMT; gene AtASMT
-
2.1.1.4 Arabidopsis thaliana Col-0 Q9T003 gene At4g35160 or AtASMT; gene AtASMT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.4 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.4 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.4 0.0000007
-
 purified recombinant enzyme, pH 7.8, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.4 additional information the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis Arabidopsis thaliana ?
-
 ?
2.1.1.4 additional information caffeic acid is no substrate Arabidopsis thaliana ?
-
 ?
2.1.1.4 additional information the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis Arabidopsis thaliana Col-0 ?
-
 ?
2.1.1.4 additional information caffeic acid is no substrate Arabidopsis thaliana Col-0 ?
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Arabidopsis thaliana Col-0 S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.4 S-adenosyl-L-methionine + serotonin enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine Arabidopsis thaliana S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?
2.1.1.4 S-adenosyl-L-methionine + serotonin enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine Arabidopsis thaliana Col-0 S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.4 ASMT
-
 Arabidopsis thaliana
2.1.1.4 N-acetylserotonin O-methyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.4 37
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.4 7.8
-
 O-methylation of N-acetylserotonin Arabidopsis thaliana
2.1.1.4 8.8
-
 O-methylation of serotonin Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.4 S-adenosyl-L-methionine
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.1.1.4 Arabidopsis thaliana AtASMT transcripts are induced by cadmium treatment in Arabidopsis thaliana followed by increased melatonin synthesis up

General Information

EC Number General Information Comment Organism
2.1.1.4 metabolism the enzyme catalyzes the conversion of N-acetylserotonin to melatonin as the last step in melatonin biosynthesis. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis Arabidopsis thaliana