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Literature summary extracted from

  • Byeon, Y.; Lee, H.Y.; Lee, K.; Back, K.
    Caffeic acid O-methyltransferase is involved in the synthesis of melatonin by methylating N-acetylserotonin in Arabidopsis (2014), J. Pineal Res., 57, 219-227.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.4 gene comt, recombinant expression in Escherichia coli strain BL21(DE3), the N-terminal His-tagged AtCOMT is more highly expressed in soluble form than the C-terminal His-tagged AtCOMT Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.4 additional information construction of an Arabidopsis thaliana comt knockout mutant (SALK_002373) with T-DNA inserted at the third exon. The comt mutant shows no detectable COMT transcript and exhibits a 20fold less COMT activity relative over the wild type, the phenotypes such as growth and fertility are indistinguishable from the wild-type plants Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.4 0.233
-
 N-acetylserotonin pH 7.8, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.4 cytoplasm
-
 Arabidopsis thaliana 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.4 additional information Arabidopsis thaliana caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines ?
-
 ?
2.1.1.4 additional information Arabidopsis thaliana Col-0 caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines ?
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin Arabidopsis thaliana
-
 S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin Arabidopsis thaliana Col-0
-
 S-adenosyl-L-homocysteine + melatonin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.4 Arabidopsis thaliana Q9T003 gene At4g35160 or AtASMT; gene AtASMT
-
2.1.1.4 Arabidopsis thaliana Col-0 Q9T003 gene At4g35160 or AtASMT; gene AtASMT
-
2.1.1.42 Arabidopsis thaliana Q9FK25
-
-
2.1.1.68 Arabidopsis thaliana Q9FK25 additionally shows flavone 3'-O-methyltransferase activity, reaction of EC 2.1.1.42, and N-acetylserotonin methyltransferase activity, reaction of EC 2.1.1.4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.4 reombinant N-and C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatograpy Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.4 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.4 additional information caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines Arabidopsis thaliana ?
-
 ?
2.1.1.4 additional information caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines Arabidopsis thaliana Col-0 ?
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Arabidopsis thaliana S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Arabidopsis thaliana Col-0 S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.42 additional information enzyme additionally shows N-acetylserotonin methyltransferase activity, reaction of EC 2.1.1.4, and caffeic acid methyltransferase activity, reaction of EC 2.1.1.68. The catalytic efficiency for N-acetylserotonin methyltransferase activity is 709fold lower than for caffeic acid methyltransferase. In vitro, N-acetylserotonin methyltransferase activity is dramatically decreased by the addition of caffeic acid in a dose-dependent manner, but the activity of caffeic acid methyltransferase is not altered by N-acetylserotonin Arabidopsis thaliana ?
-
 ?
2.1.1.68 additional information enzyme additionally shows flavone 3'-O-methyltransferase activity, reaction of EC 2.1.1.42, and N-acetylserotonin methyltransferase activity, reaction of EC 2.1.1.4. The catalytic efficiency for N-acetylserotonin methyltransferase activity is 709fold lower than for caffeic acid methyltransferase. In vitro, N-acetylserotonin methyltransferase activity is dramatically decreased by the addition of caffeic acid in a dose-dependent manner, but the activity of caffeic acid methyltransferase is not altered by N-acetylserotonin Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.4 ASMT
-
 Arabidopsis thaliana
2.1.1.4 N-acetylserotonin methyltransferase
-
 Arabidopsis thaliana
2.1.1.42 OMT1
-
 Arabidopsis thaliana
2.1.1.68 OMT1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.4 37
-
 both recombinant N- and C-terminal fusions of enzyme AtCOMT exhibit the highest enzyme activities at pH 37°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.4 7.8
-
 both recombinant N- and C-terminal fusions of enzyme AtCOMT exhibit the highest enzyme activities at pH 7.8 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.4 6.5 8.8 both recombinant N- and C-terminal fusions of the enzyme, no activity at pH 5.4 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.4 S-adenosyl-L-methionine
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.1.1.4 metabolism in vivo role played by the COMT for biosynthesizing melatonin Arabidopsis thaliana
2.1.1.42 physiological function an Arabidopsis thaliana knockout mutant exhibits less production of melatonin than the wild type when leaves are infiltrated with 1 mM N-acetylserotonin Arabidopsis thaliana
2.1.1.68 physiological function an Arabidopsis thaliana knockout mutant exhibits less production of melatonin than the wild type when leaves are infiltrated with 1 mM N-acetylserotonin Arabidopsis thaliana