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Literature summary extracted from

  • Park, S.; Byeon, Y.; Kim, Y.S.; Back, K.
    Kinetic analysis of purified recombinant rice N-acetylserotonin methyltransferase and peak melatonin production in etiolated rice shoots (2013), J. Pineal Res., 54, 139-144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.4 tryptamine activates by 25% Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.4 gene OsASMT, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged and of nontagged enzyme in Escherichia coli strain BL21(DE3), expression of N- or C-terminally His-tagged enzyme is not successful Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.4 additional information various amine compounds, including tyramine, tryptamine, N-acetyltryptamine, and 5-methoxytryptamine show no or poor inhibition of the enzyme. The enzyme does not show substrate inhibition at high N-acetylserotonin concentrations Oryza sativa
2.1.1.4 tryptophan about 30% inhibition Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.4 0.864
-
N-acetylserotonin recombinant enzyme, pH not specified in the publication, 30°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.4 cytoplasm
-
Oryza sativa 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.4 additional information ASMT-specific activity is not altered by the addition of EDTA or the divalent cation Mg2+, suggesting that Oryza sativa ASMT is a cation-independent O-methyltransferase Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin Oryza sativa
-
S-adenosyl-L-homocysteine + melatonin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.4 Oryza sativa
-
cv. Dongjin, gene osASMT
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.4 seedling
-
Oryza sativa
-
2.1.1.4 shoot
-
Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.4 0.000007
-
purified GST-tagged recombinant enzyme, pH not specified in the publication, 30°C Oryza sativa
2.1.1.4 0.000013
-
purified nontagged recombinant enzyme, pH not specified in the publication, 30°C Oryza sativa
2.1.1.4 0.00003
-
purified nontagged recombinant enzyme, pH not specified in the publication, 30°C, at 10 mM substrate N-acetylserotonin concentration Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
Oryza sativa S-adenosyl-L-homocysteine + melatonin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.4 N-acetylserotonin methyltransferase
-
Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.4 55
-
-
Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.4 S-adenosyl-L-methionine
-
Oryza sativa