BRENDA - Enzyme Database

Expression of the gene for the DELTA9 acyl-lipid desaturase in the thermophilic cyanobacterium

Kiseleva, L.L.; Serebriiskaya, T.S.; Horvath, I.; Vigh, L.; Lyukevich, A.A.; Los, D.A.; J. Mol. Microbiol. Biotechnol. 2, 331-338 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.28
expressed in Escherichia coli
Thermosynechococcus vulcanus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.28
32000
-
x * 32000, SDS-PAGE
Thermosynechococcus vulcanus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Thermosynechococcus vulcanus
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.28
Thermosynechococcus vulcanus
Q9ZAP7
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
734509
Thermosynechococcus vulcanus
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 32000, SDS-PAGE
Thermosynechococcus vulcanus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.28
expressed in Escherichia coli
Thermosynechococcus vulcanus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.28
32000
-
x * 32000, SDS-PAGE
Thermosynechococcus vulcanus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Thermosynechococcus vulcanus
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
734509
Thermosynechococcus vulcanus
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 32000, SDS-PAGE
Thermosynechococcus vulcanus
Expression
EC Number
Organism
Commentary
Expression
1.14.19.28
Thermosynechococcus vulcanus
a low level of the desC mRNA is detected in cells grown at 55°C. An about 10fold increase is observed in the levels of the transcript and the protein during the shift in temperature from 55 to 45°C. At 35°C the amount of the desC mRNA and of the enzyme accumulated in the cells, is 3 to 4times smaller than at 45°C
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.19.28
Thermosynechococcus vulcanus
a low level of the desC mRNA is detected in cells grown at 55°C. An about 10fold increase is observed in the levels of the transcript and the protein during the shift in temperature from 55 to 45°C. At 35°C the amount of the desC mRNA and of the enzyme accumulated in the cells, is 3 to 4times smaller than at 45°C
up