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Literature summary extracted from

  • Ertan, H.; Cassel, C.; Verma, A.; Poljak, A.; Charlton, T.; Aldrich-Wright, J.; Omar, S.; Siddiqui, K.; Cavicchioli, R.
    A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity (2015), J. Mol. Catal. B, 113, 1-8.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Ca2+ Ca2+ leads to an increase in protease productivity by 300% Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Pseudomonas sp.
-
-
-
3.4.24.40 Pseudomonas sp. RC
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 ammonium sulfate precipitation, anion exchange column chromatography, and gel filtration Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 azocasein + H2O
-
Pseudomonas sp. ?
-
?
3.4.24.40 azocasein + H2O
-
Pseudomonas sp. RC ?
-
?
3.4.24.40 Bovine serum albumin + H2O the enzyme cleaves 61 unique sequence pairs of bovine serum albumin involving all 20 amino acids Pseudomonas sp. ?
-
?
3.4.24.40 Bovine serum albumin + H2O the enzyme cleaves 61 unique sequence pairs of bovine serum albumin involving all 20 amino acids Pseudomonas sp. RC ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.40 AMP-P
-
Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.40 36
-
-
Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 42 48 the enzyme shows a half-life of 4.9 min, 3.4 min, 2.2 min, and 1.2 min at 42°C, 44°C, 46°C, and 48°C, respectively Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.40 0.045
-
azocasein at pH 6.5 and 25°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 6.5
-
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.40 5.2 8.8 more than 90% of maximal activity between pH 6.0 and 8.8, 44% activity at pH 5.2 Pseudomonas sp.