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Literature summary extracted from

  • Dwivedi, U.; Gupta, P.; Pandey, V.; Singh, S.; Singh, R.
    Caffeic acid O-methyltransferase from Leucaena leucocephala: Cloning, expression, characterization and molecular docking analyses (2014), J. Mol. Catal. B, 106, 63-70.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expression in Escherichia coli Leucaena leucocephala

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.0021
-
 S-adenosyl-L-methionine pH 8.0, 37°C Leucaena leucocephala
2.1.1.68 0.22
-
 3,4-dihydroxy-trans-cinnamate pH 8.0, 37°C Leucaena leucocephala

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.68 39839
-
 2 * 40000, SDS-PAGE, 2 * 39839, calculated Leucaena leucocephala
2.1.1.68 40000
-
 2 * 40000, SDS-PAGE, 2 * 39839, calculated Leucaena leucocephala
2.1.1.68 80000
-
 gel filtration Leucaena leucocephala

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Leucaena leucocephala A7U0E5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.68 0.45
-
 pH 8.0, 37°C Leucaena leucocephala

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 additional information molecular docking of potential substrates shows that the binding energy decreases in the order: thiol ester substrates, free acid substrates, alcoholic andaldehyde substrates, suggesting the best binding for thiol ester substrates Leucaena leucocephala ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Leucaena leucocephala S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.68 dimer 2 * 40000, SDS-PAGE, 2 * 39839, calculated Leucaena leucocephala

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.68 37
-
-
 Leucaena leucocephala

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.68 8
-
-
 Leucaena leucocephala

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.68 Leucaena leucocephala calculated
-
 5.6