EC Number | Crystallization (Comment) | Organism |
---|---|---|
7.4.2.5 | to 1.9 A resolution. Structure represents an intermediate state during the transition of the clamp from an open to a closed conformation. Closure of the clamp occurs in two phases, an initial movement of polypeptide cross-linking domain PPXD, helical scaffold domain HSD, and helical wing domain HWD as a unit, followed by a movement of PPXD alone toward nucleotide-binding domain NBD2. The substrate associates with the back of the clamp by dynamic hydrogen bonding and the clamp is laterally closed by a conserved loop of the PPXD | Thermotoga maritima |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.4.2.5 | E485R/E488R/R367E | mutation leads to a closed conformation of the clamp, the C-loop remains inside the clamp | Thermotoga maritima |
7.4.2.5 | R367E | mutation of a conserved residue, results in significant lateral opening of the clamp, which leads to increased dissociation of the substrate | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.4.2.5 | Thermotoga maritima | Q9X1R4 | - |
- |
7.4.2.5 | Thermotoga maritima DSM 3109 | Q9X1R4 | - |
- |