Literature summary extracted from

Choi, C.W.; An, H.Y.; Lee, Y.J.; Lee, Y.G.; Yun, S.H.; Park, E.C.; Hong, Y.; Kim, G.H.; Park, J.E.; Baek, S.J.; Kim, H.S.; Kim, S.I.
Characterization of Streptococcus pneumoniae N-acetylglucosamine-6-phosphate deacetylase as a novel diagnostic marker (2013), J. Microbiol., 51, 659-664.

Application

EC Number	Application	Comment	Organism
3.5.1.25	diagnostics	the enzyme has potential as a diagnostic marker for Streptococcus pneumoniae because of its high immunogenicity and immunospecificity	Streptococcus pneumoniae

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.1.25	recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain C43(DE3)	Streptococcus pneumoniae

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.5.1.25	cytosol	-	Streptococcus pneumoniae	5829	-
3.5.1.25	extracellular	-	Streptococcus pneumoniae	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.25	41670	-	x * 41670, sequence calculation, x * 45498, recombinant His-tagged enzyme, mass spectrometry	Streptococcus pneumoniae
3.5.1.25	45498	-	x * 41670, sequence calculation, x * 45498, recombinant His-tagged enzyme, mass spectrometry	Streptococcus pneumoniae

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.25	Streptococcus pneumoniae	Q8DN83	-	-
3.5.1.25	Streptococcus pneumoniae BAA-255	Q8DN83	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.5.1.25	additional information	the enzyme does not undergo posttranslational modification	Streptococcus pneumoniae

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.25	recombinant His-tagged enzyme from Escherichia coli strain C43(DE3) by nickel affinity chromatography and dialysis	Streptococcus pneumoniae

Subunits

EC Number	Subunits	Comment	Organism
3.5.1.25	?	x * 41670, sequence calculation, x * 45498, recombinant His-tagged enzyme, mass spectrometry	Streptococcus pneumoniae
3.5.1.25	More	the enzyme contains four internal disulfide bonds, Cys246, Cys256, Cys274, and Cys350 that may contribute to the formation of six internal disulfide bridges. Tryptic peptide mapping of the enzyme, overview	Streptococcus pneumoniae

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.1.25	NagA	-	Streptococcus pneumoniae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)