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Literature summary extracted from

  • Kim, C.; Lee, D.; Lee, C.; Ahn, I.
    Dityrosine-based substrates for the selective and sensitive assay of thermolysin (2015), J. Ind. Eng. Chem., 21, 248-253.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 additional information
-
additional information Michaelis-Menten kinetics, overview Bacillus thermoproteolyticus
3.4.24.27 0.00191
-
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.00395
-
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.27 CaCl2 required Bacillus thermoproteolyticus
3.4.24.27 NaCl required Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 additional information synthesis and evaluation of substrates useful for the selective and sensitive assay of thermolysin, overview Bacillus thermoproteolyticus ?
-
?
3.4.24.27 N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 + H2O
-
Bacillus thermoproteolyticus N,N'-diBoc-dityrosyl + 2 Ile-isoniazid
-
?
3.4.24.27 N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 + H2O
-
Bacillus thermoproteolyticus N,N'-diBoc-dityrosyl + 2 Phe-isoniazid
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.27 37
-
assay at Bacillus thermoproteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.27 0.0463
-
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus
3.4.24.27 0.238
-
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.27 7.5
-
assay at Bacillus thermoproteolyticus

General Information

EC Number General Information Comment Organism
3.4.24.27 physiological function thermolysin family proteases are related to various diseases such as bacterial infections, cholera, gastritis and peptic ulcers, and gastric carcinoma Bacillus thermoproteolyticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.27 24.3
-
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus
3.4.24.27 60.3
-
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2 pH 7.5, 37°C Bacillus thermoproteolyticus