Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chang, T.; Huang, Y.; Ou, Y.
    The role of ginsenosides in inhibiting ubiquitin activating enzyme (E1) activity (2014), J. Funct. Foods, 7, 462-470.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.45 ginsenoside Rb1 increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
6.2.1.45 ginsenoside Rb2 increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
6.2.1.45 ginsenoside Rc increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
6.2.1.45 ginsenoside Rd increases ubiquitination on E1 enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens

Application

EC Number Application Comment Organism
6.2.1.45 medicine functional foods, e.g. ginsenoside Rg1 in Panax ginseng root, targeting the ubiquitin-activating enzyme (E1) of the ubiquitin-proteasome pathway as anti-tumour agents are considered to be important for cancer chemoprevention Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.45 recombinant expression of mRFP-enzyme in Escherichia coli strain DH5alpha, linking of ubiquitin to the C-terminus of RFP through a peptide bond Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.45 ginsenoside Re inhibits ubiquitin-activating enzyme, from Panax ginseng roots, a traditional herbal medicine or food Homo sapiens
6.2.1.45 ginsenoside Rg1 inhibit ubiquitin-activating enzyme, from Panax ginseng roots, a traditional herbal medicine or food, causes 89.2% inhibition at 0.05 mM Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.45 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.45 42000
-
 x * 42000, recombinant mRFP-enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Homo sapiens E1-activating enzyme activates ubiquitin via an adenylated intermediate and catalyzes its transfer to an E2 enzyme AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.45 Homo sapiens
-
 eight human E1s
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.45 recombinant mRFP-enzyme from Escherichia coli strain DH5alpha by combined anion exchange/affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine E1-activating enzyme activates ubiquitin via an adenylated intermediate and catalyzes its transfer to an E2 enzyme Homo sapiens AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine E1 activates ubiquitin or an ubiquitin-like protein and transfers it to the E2-conjugating enzyme Homo sapiens AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.45 ? x * 42000, recombinant mRFP-enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.2.1.45 E1
-
 Homo sapiens
6.2.1.45 ubiquitin activating enzyme
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.45 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.45 7.6
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.45 ATP dependent on Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.2.1.45 0.0035
-
 recombinant enzyme, pH 7.6, 37°C Homo sapiens ginsenoside Rg1
6.2.1.45 0.0628
-
 recombinant enzyme, pH 7.6, 37°C Homo sapiens ginsenoside Re