Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Byeon, Y.; Choi, G.H.; Lee, H.Y.; Back, K.
    Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice (2015), J. Exp. Bot., 66, 6917-6925.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.4 gene OsCOMT, recombinant His6-tagged enzyme expression in Escherichia coli strain BL21(DE3), generation of transgenic Nicotiana benthamiana plants and Oryza sativa plants expressing the enzyme using the Agrobacterium tumefaciens strains GV2260 and LBA4404 transfection method Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.4 caffeic acid in vitro N-acetylserotonin methyltransferase activity is significantly and competitively inhibited by caffeic acid, a substrate of the caffeic acid O-methyltransferase, 73% inhibition at 0.01 mM, 93% at 0.1 mM Oryza sativa
2.1.1.4 quercetin in vitro N-acetylserotonin methyltransferase activity is significantly and competitively inhibited by quercetin, a substrate of the caffeic acid O-methyltransferase, in vivo production of melatonin is significantly inhibited by quercetin in 4-week-old detached rice leaves, 97% inhibition at 0.01 mM, complete inhibition at over 0.05 mM Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.4 additional information
-
 additional information Michaelis-Menten kinetics Oryza sativa
2.1.1.4 0.243
-
 N-acetylserotonin pH 7.8, 30°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.4 cytoplasm
-
 Oryza sativa 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.4 additional information Oryza sativa caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. The ASMT activity (Vmax/Km) of OsCOMT is 609fold higher than that of rice ASMT1 ?
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin Oryza sativa
-
 S-adenosyl-L-homocysteine + melatonin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.4 Oryza sativa Q6ZD89 cf. EC 2.1.1.42
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.4 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.4 leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.4 additional information caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. The ASMT activity (Vmax/Km) of OsCOMT is 609fold higher than that of rice ASMT1 Oryza sativa ?
-
 ?
2.1.1.4 S-adenosyl-L-methionine + N-acetylserotonin
-
 Oryza sativa S-adenosyl-L-homocysteine + melatonin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.4 ASMT
-
 Oryza sativa
2.1.1.4 N-acetylserotonin methyltransferase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.4 37
-
 assay at Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.4 7.8
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.4 S-adenosyl-L-methionine
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
2.1.1.4 malfunction melatonin production is significantly reduced or increased when the rice plants overexpressed or suppressed the endogenous rice caffeic acid O-methyltransferase Oryza sativa
2.1.1.4 malfunction transgenic rice plants overexpressing rice caffeic acid O-methyltransferase/N-acetylserotonin O-methyltransferase show an increase in melatonin levels whereas transgenic rice plants suppressing the rice caffeic acid O-methyltransferase/N-acetylserotonin O-methyltransferase have a significant decrease on melatonin levels Oryza sativa
2.1.1.4 physiological function direct role of caffeic acid O-methyltransferase/N-acetylserotonin O-methyltransferase in melatonin biosynthesis in plants Oryza sativa