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Literature summary extracted from

  • Kawaguchi, M.; Yasumasu, S.; Shimizu, A.; Kudo, N.; Sano, K.; Iuchi, I.; Nishida, M.
    Adaptive evolution of fish hatching enzyme: one amino acid substitution results in differential salt dependency of the enzyme (2013), J. Exp. Biol., 216, 1609-1615.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B25 expressed in Escherichia coli BL21 (DE3) pLysE cells Oryzias latipes
3.4.24.B25 expressed in Escherichia coli BL21 (DE3) pLysE cells Fundulus heteroclitus

General Stability

EC Number General Stability Organism
3.4.24.B25 isoform HCE1 shows the highest activity in 0 mol/l NaCl, while isoform HCE2 shows the highest activity in 0.125 mol/l NaCl Fundulus heteroclitus
3.4.24.B25 optimal salinity of the enzyme is found in 0 mol/l NaCl, and activity decreases with increasing salt concentrations Oryzias latipes
3.4.24.B25 optimal salinity of the enzyme is found in 0 mol/l NaCl, and activity decreases with increasing salt concentrations Fundulus heteroclitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B25 egg envelope protein + H2O Oryzias latipes
-
 ?
-
 ?
3.4.24.B25 egg envelope protein + H2O Fundulus heteroclitus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B25 Fundulus heteroclitus Q587K9
-
-
3.4.24.B25 Oryzias latipes Q14TH9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B25 embryo
-
 Oryzias latipes
-
3.4.24.B25 embryo
-
 Fundulus heteroclitus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B25 casein + H2O
-
 Oryzias latipes ?
-
 ?
3.4.24.B25 casein + H2O
-
 Fundulus heteroclitus ?
-
 ?
3.4.24.B25 egg envelope protein + H2O
-
 Oryzias latipes ?
-
 ?
3.4.24.B25 egg envelope protein + H2O
-
 Fundulus heteroclitus ?
-
 ?
3.4.24.B25 PSKRPEAPGVP + H2O
-
 Fundulus heteroclitus PSKRPE + APGVP
-
 ?
3.4.24.B25 QKQTPSYPQQPQ + H2O
-
 Fundulus heteroclitus QKQTPS + YPQQPQ
-
 ?
3.4.24.B25 Z-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O best substrate Oryzias latipes Z-Leu-Leu-Glu + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B25 hatching enzyme
-
 Oryzias latipes
3.4.24.B25 hatching enzyme
-
 Fundulus heteroclitus
3.4.24.B25 HCE
-
 Oryzias latipes
3.4.24.B25 HCE1
-
 Fundulus heteroclitus
3.4.24.B25 HCE2
-
 Fundulus heteroclitus
3.4.24.B25 High choriolytic enzyme
-
 Oryzias latipes
3.4.24.B25 High choriolytic enzyme
-
 Fundulus heteroclitus