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Literature summary extracted from
- The efficient expression of human fibroblast collagenase in Escherichia coli and the discovery of flavonoid inhibitors (2013), J. Enzyme Inhib. Med. Chem., 28, 741-746.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.24.7 | synthesis | improved method for high-level expression of soluble human MMP-1 catalytic domain in Escherichia coli | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.7 | expression in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.7 | fisetin | mixed-type inhibition | Homo sapiens |  |
| 3.4.24.7 | quercetin | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.7 | 0.013 | - |
2,4-dinitrophenyl-Pro-beta-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(N-methyl-2-aminobenzoyl)-NH2 | pH 7.5, 40°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.7 | Ca2+ | optimum concentration 5 mM | Homo sapiens | |
| 3.4.24.7 | Zn2+ | in presence of 5 mM Ca2+, optimum concentration 0.001 mM, decrease in activity above | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.7 | 21000 | - |
x * 21000, SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.7 | Homo sapiens | P03956 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.7 | fibroblast | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.7 | 2,4-dinitrophenyl-Pro-beta-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(N-methyl-2-aminobenzoyl)-NH2 + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.7 | ? | x * 21000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.7 | mmp1 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.7 | 40 | - |
- |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.7 | 7.5 | - |
- |
Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.7 | 0.00135 | - |
fisetin | pH 7.5, 40°C | Homo sapiens | |
| 3.4.24.7 | 0.00149 | - |
quercetin | pH 7.5, 40°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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