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Literature summary extracted from

  • Hu, H.; Hu, L.; Yu, Z.; Chasse, A.E.; Chu, F.; Li, Z.
    An orphan kinesin in trypanosomes cooperates with a kinetoplastid-specific kinesin to maintain cell morphology by regulating subpellicular microtubules (2012), J. Cell Sci., 125, 4126-4136.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.3 gene Tb927.11.10760 Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
5.6.1.3 additional information RNAi knockdown 15 orphan kinesins in procyclic trypanosomes, RNAi of orphan kinesin TbKIN-D results in severe growth inhibition and cell death after 4 days, TbKIN-D mRNA is significantly depleted after RNAi for 2 days, and TbKIN-D protein is gradually depleted from the cells after RNAi impairing organelle segregation, while synthesis of the flagellum or flagellum attachment zone are not affected Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.1.3 microtubule orphan kinesin TbKIN-D associates with cytoskeletal microtubules and is distributed throughout the cytoskeleton at all cell cycle stages Trypanosoma brucei 5874
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.3 Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.3 ATP + H2O + a kinesin associated with a microtubule at position n Trypanosoma brucei
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 ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
5.6.1.3 additional information Trypanosoma brucei enzyme TbKIN-D associates with cytoskeletal microtubules in vivo, and TbKIN-D interacts with TbKIN-C, a kinetoplastid-specific kinesin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.3 Trypanosoma brucei Q383K3 gene Tb927.11.10760
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Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.3 copurification of orphan kinesin TbKIN-D and kinetoplastid-specific kinesin TbKIN-C by tandem affinity purification in a two-step chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.3 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Trypanosoma brucei ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
5.6.1.3 ATP + H2O + a kinesin associated with a microtubule at position n in vitro ATPase activity of the purified motor domain of TbKIN-D Trypanosoma brucei ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?
5.6.1.3 additional information enzyme TbKIN-D associates with cytoskeletal microtubules in vivo, and TbKIN-D interacts with TbKIN-C, a kinetoplastid-specific kinesin Trypanosoma brucei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.3 orphan kinesin
-
 Trypanosoma brucei
5.6.1.3 TbKIN-D
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
5.6.1.3 malfunction depletion of TbKIN-D disrupts cell morphology, resulting in elongation of the posterior of the cell, which is filled with newly assembled microtubules. RNAi of TbKIN-D impairs organelle segregation and leads to severe growth inhibition and cell death, TbKIN-D deficiency significantly interferes with cytoskeletal microtubules, phenotype Trypanosoma brucei
5.6.1.3 additional information enzyme TbKIN-D possesses an N-terminal motor domain and three coiled-coil motifs in the C-terminus. Although the microtubule-binding motif in the motor domain of TbKIN-D is conserved, the putative nucleotide-binding motif lacks the well-conserved lysine residue in the P-loop and two additional conserved motifs, SSRSH (switch I) and DLAGSE (switch II) Trypanosoma brucei
5.6.1.3 physiological function microtubules are a vital part of the cytoskeleton of eukaryotic cells and are involved in various cellular processes. The cytoskeleton of Trypanosoma brucei is characterized by an array of subpellicular microtubules and is essential for maintenance of cell shape and polarity, the orphan kinesin TbKIN-D in trypanosomes cooperates with a kinetoplastid-specific kinesin to maintain cell morphology by regulating subpellicular microtubules. The orphan kinesin TbKIN-D regulates the organization of subpellicular microtubules and is required for maintaining cell morphology, and TbKIN-D possesses in vitro ATPase activity and associates with cytoskeletal microtubules. Enzyme TbKIN-D is essential for cell proliferation and viability Trypanosoma brucei