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Literature summary extracted from

  • Peng, H.M.; Liu, J.; Forsberg, S.E.; Tran, H.T.; Anderson, S.M.; Auchus, R.J.
    Catalytically relevant electrostatic interactions of cytochrome P450c17 (CYP17A1) and cytochrome b5 (2014), J. Biol. Chem., 289, 33838-33849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.19 expressed in Escherichia coli JM109 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.19 R347K the mutant exhibits similar 17-hydroxylase and b5-stimulated 17,20-lyase activity as the wild type enzyme Homo sapiens
1.14.14.19 R358K the mutant exhibits similar 17-hydroxylase and b5-stimulated 17,20-lyase activity as the wild type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.19 additional information Homo sapiens the enzyme also shows 17,20-lyase activity which is stimulated by cytochrome b5 ?
-
 ?
1.14.14.19 pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.19 Homo sapiens P05093
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.19 nickel-nitrilotriacetic acid affinity resin column chromatography, His Select resin column chromatography, and Sartorius Vivapure-S spin column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.19 additional information the enzyme also shows 17,20-lyase activity which is stimulated by cytochrome b5 Homo sapiens ?
-
 ?
1.14.14.19 pregnenolone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.19 CYP17A1
-
 Homo sapiens
1.14.14.19 cytochrome P450c17
-
 Homo sapiens