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Literature summary extracted from

  • Saen-Oon, S.; Lee, S.G.; Jez, J.M.; Guallar, V.
    An alternative mechanism for the methylation of phosphoethanolamine catalyzed by Plasmodium falciparum phosphoethanolamine methyltransferase (2014), J. Biol. Chem., 289, 33815-33825.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.103 D128A mutant in complex with S-adenosylhomocysteine and either phosphoethanolamine or phosphocholine, hanging drop vapor diffusion method, using 20% (w/v) PEG 8000, 0.1 M sodium cacodylate (pH 6.5), 0.2 M sodium acetate, 20 mM tris(2-carboxyethyl)phosphine, and 5 mM S-adenosyl-L-homocysteine Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.103 D128A compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 91% activity) Plasmodium falciparum
2.1.1.103 D128E compared to the wild type enzyme, the mutant shows about 90% loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 182% activity) Plasmodium falciparum
2.1.1.103 D128N compared to the wild type enzyme, the mutant shows complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 24% activity) Plasmodium falciparum
2.1.1.103 D128Q compared to the wild type enzyme, the mutant shows almost complete loss of activity with phosphoethanolamine but not with the final substrate of the methylation pathway (about 89% activity) Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.054
-
 Ethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.181
-
 N,N-dimethylethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.773
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.786
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 7.99
-
 Ethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 21.17
-
 Ethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Plasmodium falciparum
-
 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Plasmodium falciparum Q6T755
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.103 nickel-affinity column chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + N,N-dimethylethanolamine phosphate
-
 Plasmodium falciparum S-adenosyl-L-homocysteine + phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.103 phosphoethanolamine methyltransferase
-
 Plasmodium falciparum
2.1.1.103 PMT
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.103 0.005
-
 Ethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.022
-
 Ethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.367
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 1.483
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 1.82
-
 Ethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 3.08
-
 N,N-dimethylethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum

General Information

EC Number General Information Comment Organism
2.1.1.103 physiological function the enzyme is essential for normal growth and development Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.103 0.00025
-
 Ethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.00271
-
 Ethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 0.474
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128N, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 1.887
-
 N,N-dimethylethanolamine phosphate mutant enzyme D128A, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 17.04
-
 N,N-dimethylethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum
2.1.1.103 33.64
-
 Ethanolamine phosphate wild type enzyme, at pH 8.0 and 20°C Plasmodium falciparum