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Literature summary extracted from

  • Petrunak, E.M.; DeVore, N.M.; Porubsky, P.R.; Scott, E.E.
    Structures of human steroidogenic cytochrome P450 17A1 with substrates (2014), J. Biol. Chem., 289, 32952-32964.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.19 expressed in Escherichia coli JM109 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.19 mutant enzyme A105L in complex with progesterone, pregnenolone, 17alpha-hydroxypregnenolone, or abiraterone is crystallized by the hanging drop vapor diffusion method using 100 mM Tris-HCl, pH 8.5, 25% (w/v) PEG 4000, 150 mM magnesium chloride hexahydrate, and 4-6% (v/v) glycerol. Mutant enzyme A105L in complex with 17alpha-hydroxyprogesterone is crystallized by the hanging drop vapor diffusion method using 175 mM Tris-HCl, pH 8.5, 30% (w/v) PEG 3350, 250 mM lithium sulfate, and 3% (v/v) glycerol Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.19 A105L the effect of the A105L mutation is to increase progesterone affinity at least 2fold but the mutant has little effect on the already lower affinity for either progesterone hydroxylation product Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.19 abiraterone
-
 Homo sapiens
1.14.14.19 TOK-001
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.19 0.00038
-
 pregnenolone mutant enzyme A105L, at pH 7.4 and 37°C Homo sapiens
1.14.14.19 0.00093
-
 pregnenolone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1.14.14.19 0.00196
-
 progesterone mutant enzyme A105L, at pH 7.4 and 37°C Homo sapiens
1.14.14.19 0.0105
-
 progesterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.19 a C21-steroid + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 a 17alpha-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.19 additional information Homo sapiens a minor conformation may yield the minor 16alpha-hydroxyprogesterone metabolite from progesterone ?
-
 ?
1.14.14.19 pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.19 Homo sapiens P05093
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.19 nickel-nitrilotriacetic acid-agarose resin column chromatography, carboxymethyl-Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.19 a C21-steroid + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens a 17alpha-hydroxy-C21-steroid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.19 additional information a minor conformation may yield the minor 16alpha-hydroxyprogesterone metabolite from progesterone Homo sapiens ?
-
 ?
1.14.14.19 pregnenolone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.19 progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.19 CYP17A1
-
 Homo sapiens
1.14.14.19 steroidogenic cytochrome P450 17A1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.19 0.0065
-
 pregnenolone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1.14.14.19 0.0065
-
 pregnenolone mutant enzyme A105L, at pH 7.4 and 37°C Homo sapiens
1.14.14.19 0.017
-
 progesterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1.14.14.19 0.019
-
 progesterone mutant enzyme A105L, at pH 7.4 and 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.19 heme
-
 Homo sapiens